Item No. 1 of 1

INVESTIGATOR: Burrack, H. J.

PERFORMING INSTITUTION:
NORTH CAROLINA STATE UNIV
RALEIGH, NORTH CAROLINA 27695

SUSTAINABLE STRATEGIES TO MANAGE SPOTTED WIND DROSOPHILA IN UNITED STATES FOOD CROPS.

CLASSIFICATION HEADINGS: R211 . Insects, Mites, and Other Arthropods Affecting Plants; S1129 . Berries and cane fruits, general/other; F1130 . Entomology and acarology; R215 . Biological Control of Pests Affecting Plants; R216 . Integrated Pest Management Systems; F2090 . Statistics, econometrics, and biometrics

NON-TECHNICAL SUMMARY: Spotted wing drosophila (SWD, Drosophila suzukii), native to eastern Asia, is a devastating pest of soft skinned fruits and has rapidly expanded its global range in the last five years to include the United States, Canada, Mexico, Europe, and most recently South America (Walsh et al. 2011; Cini et al. 2012; Depra et al. 2014). SWD is unique among Drosophila species in that the larvae feed on sound, otherwise marketable fruit and have a wide host range including all soft fleshed cultivated and uncultivated fruit (Cini et al. 2012). The crops most significantly affected by SWD include blueberries, blackberries, raspberries, strawberries, and cherries, valued at over $4.37 billion annually (USDA NASS 2013) and grown on 41,825 farms (Census of Agriculture 2012). In addition to its broad host range, SWD has a phenomenal reproductive capacity, with the ability to complete up to 16 generations per year under optimal conditions. Female flies are capable of laying up to 350 eggs over their life span, and individual flies have been observed with up to 40 mature eggs in their ovaries at a time (Burrack et al. 2015). There is zero tolerance for SWD infested fruit for fresh market or whole frozen products, so detection of even a single larva in a shipment results in complete rejection. National crop loss potential due solely to SWD in the United States is estimated at $718 million annually, and increased costs directly related to management practices are estimated to range between $129 to 172 million annually, equivalent to 6-8% of farmgate value (Bolda et al. 2010, USDA SCRI SWD*IPM Stakeholder Meeting 2014, eFly SWD Working Group 2012). Observed crop losses due to SWD in the western US are as high as 80% (Bolda et al. 2010). In a winter 2015 survey of 436 US fruit growers in the United States, respondents from 31 states reported crop losses due to SWD estimated at over $133 million and increases in pesticide costs of between $100-$300 per acre.Because of the lack of infestation tolerance growers employ very conservative SWD management strategies, chiefly preventative insecticide sprays (Wise et al. 2014). Insecticide applications are typically made on a 5-10 day schedule, from the point at which fruit begin to ripen through the end of harvest. The risk of crop loss from SWD increases during the growing season due to increasing population pressure (Wiman et al. 2014). Growers of late season sweet cherry report that as many as 11 additional pesticide applications are required to harvest a saleable crop, and late season berries may require up to 16 additional pesticide applications. Insecticide evaluations in blueberry and raspberry indicate decline in residual performance within 5-10 days, depending on the insecticide, and rainfall is further detrimental to residual activity (Van Timmeren and Isaacs 2013). Therefore, even intense pesticide programs have been observed to fail in adverse environmental conditions. This projectaims to understand how to manage SWD across various cropping systems and climatic regions. We employ a stakeholder-driven, bottom-up approach to develop economically and environmentally sustainable SWD management practices that will reduce reliance on pesticides. Our project includes innovative, forward-looking research activities that will enable future advances in SWD management nationwide. The goals of our project are to develop sustainable SWD management programs, to implement these programs, evaluate their effects, and to identify promising innovations that will enable future enhancements of SWD management. Ours is the first project on SWD with national participation and extends the work of previously funded projects to areas where SWD had not been detected when they were awarded.

OBJECTIVES: The goals of our project are to develop sustainable SWD management programs, to implement these programs, evaluate their effects, and to identify promising innovations that will enable future enhancements of SWD management. Ours is the first project on SWD with national participation and extends the work of previously funded projects to areas where SWD had not been detected when they were awarded.Objective 1: Implement and evaluate SWD management programs1.1 Develop and implement grower-scale best management practices for SWD.1.2 Build tools to measure SWD impact, predict losses, and suggest mitigation strategies.1.3 Provide stakeholders with results, applications, and interpretation of project activities.Objective 2: Develop tactics and tools that predict SWD risk2.1 Field validate and implement population models.2.2 Determine sources of SWD populations between and during growing seasons.2.3 Develop monitoring tools that accurately estimate SWD populations and infestation risk.Objective 3: Optimize sustainable SWD management programs3.1 Reduce reliance on insecticides in management programs.3.2 Develop insecticide resistance detection, minimization, and management strategies.3.3 Discover natural enemies capable of contributing to population reduction.3.4 Reduce infestation rate in fruit post-harvest.3.5 Develop genetic management tactics.

APPROACH: Objective 1: Implement and evaluate SWD management programsObjective 1.1 Develop and implement grower-scale best management practices for SWD.IPM programs will be compared in all participating states via replicated trials consisting of a minimum of two treatments (grower standard versus SWD IPM) replicated at least three times in plots as large as feasible. At each annual stakeholder advisory board meeting, we will develop or update the crop and region specific programs to be tested. 1.2 Build tools to measure SWD impact, predict losses, and suggest mitigation strategies.1.2.1 Baseline bioeconomic modelWe will develop spatial bioeconomic models of SWD diffusion and control to identify profit-maximizing management strategies.The bioeconomic model will have two components: 1) a SWD spatial diffusion model and 2) a profit maximization economic model.1.3 Provide stakeholders with results, applications, and interpretation of project activitiesWe will conduct surveys of our target audience in Years 1 and 4 to assess SWD impact and stakeholder need (Year 1), generate data necessary for bioeconomic model development (Years 1 and 4), and to assess changes in stakeholder knowledge, skills, and practices to measure overall project impacts (Year 4). A central website will be developed and house project-specific outcomes, including stakeholder decision aids for SWD population risk assessment, impact assessment, and management decisions, annual reports, and dynamic content, including a frequently updated blog and integrated social media and feeds of dynamic blog and social media content. A webinar series, hosted at our project website, will be produced and achieved. Objective 2: Develop tactics and tools that predict SWD risk 2.1 Field validate and implement population models.We will field validate a stage-specific population model using information generated from Obj 2.2. and 2.Validation will consist of controlled experiments using caged SWD populations of known size and age subjected to variable environmental conditions.2.2 Determine sources of SWD populations between and during growing seasons.2.2.1 Fitness consequences of low and high temperaturesThe capacity of SWD to overwinter in different regions and different habitats, the timing of emergence, and resumption of reproduction will be investigated through field cage experiments conducted in CA, GA, MI, NY, ME and OR using winter-conditioned SWD.Cooperators in these same states will also investigate the importance of alternative food sources for off-season survival and reproduction. 2.2.2 Long distance movement and sources of SWDSix populations of SWD will be selected for SNP discovery. Once appropriate markers have been identified from target populations, they will be employed to determine movement and population biology of target sample populations. Samples of individual adult SWD will be collected at three time periods for two seasons.2.2.3. Non crop host use and local movement of SWDWe will also target the development of SNPs that will allow for detection of movement between environments within a state (focus in OR) and local movement within a field (focus on blackberries in NC).2.3 Develop monitoring tools that accurately estimate SWD populations. To determine the range of attraction of SWD traps, we willconduct wind tunnel experiments on currently available lures and thencompare trapping densities suggested by controlled experiments in replicated field trials.We will also seek to develop improved attractants based on SWD's unique biology using next-generation sequencing (RNA-seq) to investigate the change in the olfactory transcriptome in SWD females when they are gravid. Objective 3: Optimize sustainable SWD management programs3.1 Reduce reliance on insecticides in management programs 3.1.1 Improve insecticide update and fly controlWe will conduct replicated field trials in plantings at research stations or with grower cooperators to compare the performance of season-long insecticide programs that are untreated, treated with a regionally-relevant standard insecticide program, or the standard program with sucrose (2 pounds per 100 gallons) as a feeding stimulant. Other promising stimulants will be tested as available. 3.1.2. Reduce rainfall-mediated loss of efficacyReplicated field trials to compare the performance of insecticide treatments with and without additives that protect materials from rainfall will be conducted at research stations or with grower cooperators across this project.3.2 Develop insecticide resistance detection, minimization, and management strategies.3.2.1 Field resistance monitoringWe will develop a standard resistance screening methodology in Year 1 of our project. Following the development of standard methods, we will establish a national resistance-monitoring program with locations in CA, GA, MI, NC, NJ, and OR. If resistance is detected, cross resistance to other classes will be assessed.3.2.2 Resistance mechanism determinationThe mechanisms underlying detected (in the field) or selected (in the laboratory) resistance will be determined via biochemical assays, transcriptomics, and identification of target site mutations. 3.2.3 Characterization of economically efficient resistance management strategiesResults from resistance monitoring and mechanism analyses will be used to develop computational bioeconomic models of optimal spray frequency and chemical choice. Restuls will be used in conjunction with the baseline bioeconomic model to generategrower-level recommendations for monitoring and insecticide spray frequency.3.3 Discover natural enemies capable of contributing to population reduction.3.3.1. Determine geographic distributions and impact of resident parasitoids attacking SWDWe will evaluate the potential impact of resident parasitoids on SWD in different regions of the United States.3.3.2. Continue and enhance a classical biological control program for novel Asian SWD parasitoidsWe will explore, import and screen exotic parasitoids to determine their effectiveness and safety (non-target impact) collected viaforeign exploration to the native range of SWD and other locations in Asia it is known to occur during Years 1-4. 3.3.3. Conduct field releases of resident and/or imported parasitoids and determine their impactPetitions for release of promising species will be prepared, and if approved, parasitoids will be mass-reared for release in different regions. 3.4 Optimize post harvest practices to minimize SWD contaminationWe will focus on measuring the effect of two frequently used post-harvest practices on SWD infestation rates: optical and soft sorting and cold storage3.5 Develop genetic control tactics3.5.1. Development of transgenic SWDWe will develop transgenic male-only strains of SWD that carry two component tetracycline-repressible female-embryo dominant lethal systems. An effective strain could provide growers with an alternative, species-specific means for control of SWD populations.3.5.2 Risk assessment of transgenic SWDSub objective lead: Zachary Brown, North Carolina State UniversityWe will work with the Genetic Engineering and Society Center at NC State University to conduct a risk assessment to systematically identify hazards of the female-specific lethal transgenic SWD and evaluate the likelihood and severity of these hazards.

KEYWORDS: Spotted wing drosohila; blueberry; caneberry; cherry; invasive species

PROGRESS: 2018/09 TO 2019/08
Target Audience:The direct target audience for this project consists of growers of crops impacted by spotted wing drosophila (SWD) andthe extension personnel that serve them, as well as an indirect audience of fruit marketers and consumers. Our primary efforts are directed at US cherry, blueberry, blackberry, raspberry, and strawberry growers, but we also engage with growers of other crops that have SWD concerns. Our project team has also developed international connections tofacilitate information transfer to other parts of the world where SWD is a significant pest. Efforts to reach this target audience during this reporting period include our project website (SWDManagement.org), in person extension presentations and hands on workshops (47total to an audience of at least 1749 individuals), and two project webinars (https://swdmanagement.org/topics/webinars/) which were viewed live by 468 attendees and has been viewed as a recording 506 times. See "Products" and "Other Products" for specific meeting information. We held an annual in person meeting of our project team andadvisory board (https://swdmanagement.org/stakeholder-advisory-board/) in Raleigh, NC on 21-22 February 2019. At this meeting, we reviewed project results, planned field research for 2019, and developed objectives and methods for future collaborative projects. As a result, we submitted a SCRI proposal in 2019 (not funding) and are preparing a revision for 2020 (invited for full propopsal).In addition, we conducted quarterly update meetings via Zoom with project team and advisory board members on 28 May, 9 September, and 5 November 2019. These sessions provided project updates to advisory board members who were then encouraged to share them with their peers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Training events are listed in "Other Products". In addition to these stakeholder focused activities, we also trained junior scientists including a total of 12 individualundergraduates (7.2 FTE), 4 graduate students (3 FTE), and 10 post doctoral scientists (7.3 FTE). How have the results been disseminated to communities of interest?During this reporting period, results from our project have been shared via ourwebsite (SWDManagement.org), in person extension presentations and hands on workshops (47total to an audience of at least 1749 individuals), and two project webinars (https://swdmanagement.org/topics/webinars/) which were viewed live by 468 attendees and has been viewed as a recording 506 times. See "Products" and "Other Products" for specific meeting information. We held an annual in person meeting of our project team andadvisory board (https://swdmanagement.org/stakeholder-advisory-board/) in Raleigh, NC on 21-22 February 2019. At this meeting, we reviewed project results, planned field research for 2019, and developed objectives and methods for future collaborative projects. As a result, we submitted a SCRI proposal in 2019 (not funding) and are preparing a revision for 2020 (invited for full propopsal).In addition, we conducted quarterly update meetings via Zoom with project team and advisory board members on 28 May, 9 September, and 5 November 2019. These sessions provided project updates to advisory board members who were then encouraged to share them with their peers. What do you plan to do during the next reporting period to accomplish the goals?Impacts of this project include significant new understanding the biology of SWD in US fruit crops as described below, large scale outreach to stakeholders, and revised management recommendations that reduce reliance on insecticide use for SWD and identify issues with current management practices. Most noteably we have: imported, screened, and requested permits for promising biocontrol agents;identified emerging pesticide resistance in CA and developed methods to detect resistance development elsewhere;described overwintering biology;and developed an unparelleled resource consisting of over 350 fully sequenced genomes to enable future research. Our project will conclude in August 2020, following an approved no cost extension. During this final period, we will complete replication of field trials, product publications, hold a final project webinar, and develop a plan and funding strategy for future collaborative projects.

IMPACT: 2018/09 TO 2019/08
What was accomplished under these goals? Obj 1.1: In Georgia, farm-scale trials were repeated in rabbiteye blueberries to compare season-long SWD management programs based on farmer's management practices (FMP) and the site-specific best management practices (BMP). FMP program included weekly applications rotating between Malathion and Mustang Maxx and the BMP program included applications of Malathion- Delegate-Grandevo (biopesticide)-Exirel-Grandevo. Based on results of semi-field bioassays, BMP provided control equivalent to the FMP. In Maine, collaborators investigated spatial dynamics of fruit infestation by spotted wing drosophila in three fields which varied greatly in their infestation level (0.7 - 4.1maggots per sample). Within field variance was quite high and increased as the mean infestation increased. Spatial heat maps of the maggot infestation in each of the three fields were constructed using kriging. This preliminary study suggests that infestation is concentrated at the field edge and decreases as one moves out into the field. Obj: 1.2: Lead by team members at Cornell, we have focused on three production systems this reporting period. Michigan blueberries, bioeconomic model: Preliminary results show that growers should pay close attention to the amount of female adult SWD captured using traps and regard this as an early alert of the SWD activities. Preliminary results also suggest that once over half of the field is found to be infested by fruit sampling, a more economical decision is to discard the field rather than investing in more controls. North Carolina blueberries- Preliminary results show that using one treatment is not an optimal strategy. It would be better to change the treatments according to different weeks and different numbers of captured SWD. Maine blueberries- This project has produced an interactive decision tool for extension personnel and growers in managing SWD (See "Products". Obj1.3: Described below and in "Target Audience". Obj 2.1: We conducted controlled field cage experiments in MI, GA, NC, and OR to validate existing stage specific population models and test implications of different pesticide use patterns. Obj: 2.2: Team members in Oregon began to develop methods to mark and track SWD based on volatilized fatty acids associated with larval diet. Team members in the Chiu Laboratory at UC Davis have performed extensive molecular and biochemical experiments to investigate the mechanisms by which Drosophila species, including Drosophila suzukii, interpret temperature and photoperiodic signals and regulate seasonal physiology. The manuscript describing this data are currently in revision in PNAS. This manuscript is significant as it will help to predict the seasonal physiology and population size of SWD based on climatic data. The Chui lab has also finished sequencing genomes from SWD collected over time in several colder U.S. states as compared to southern states. Bioinformatic analysis is currently in progress to determine if SWD overwinters in Northern states and has adapted to colder climates. Overall, we have sequenced over 350 SWD genomes. This will be a valuable data set for studying invasion genomics, local adaptations and bottlenecks, development of insecticide resistance, and for the design of genetic pest control strategies that can be effective against SWD from a broad range of geographical locations. Obj 2.3: The Syed Laboratory at the University of Kentucky has identified a blend of two chemicals from SWD attractive yeasts and tested them in the field where they appear to have significant potential. The Gut Laboratory at Michigan State also identified volatiles were collected from two yeast species, Hanseniaspora uvarum and Candid zemplinina, and from raspberry fruit. In laboratory bioassays, volatiles emitted from H. uvarum captured the highest number of male and female SWD, followed by C. zemplinina, raspberry, and an unbaited control. In the field trial, SWD captures were low and equivalent regardless of the type of attractant. Translating behavioral responses in the lab to effects in the field continues to be the biggest challenge in our search for an effective attractant for use in monitoring or as an attract-and-kill program for SWD. The Gut lab continued efforts to develop a sticky panel trap as an alternative to the standard deli-cup trap design for SWD. Seven rectangular plastic panel traps were custom-made in various colors and patterns, but during the growing season, the standard cup trap baited with a Scentry lure was the most effective. However, post harvest all the panel traps captured significantly more flies than the cup trap. The green trap with the purple dot was the most effective panel trap. Obj 3.1: Collaborators at Michigan State repeated small plot trials testing multiple timings using the newer insecticide cyclaniliprole (Harvanta SL) in cherries. The aim was to refine how best to time the use of excellent compounds available for managing SWD. There was good SWD pressure in the plots and the results revealed that a spray program starting no later than 21 days before harvest with 3 applications provided excellent control but starting one week earlier was unnecessary. Obj 3.2: Based on previous year's results from GA and MI, we revised the diagnostic dose bioassay protocols. During 2019 field-season, we used LC90x8 as a diagnostic dose to quickly screen field-collected flies for resistance. Both diagnostic-dose and full-range bioassays were conducted on 2018 field populations. Several field populations were collected in 2019 and bioassays are underway with results to be published in 2020. The Dong Laboratory at Michigan State University has evaluated repellency and toxicity of plant-derived compounds against adults of SDW in various bioassays. Several compounds are potent repellents against SWD by targeting specific olfactory receptors. We also identified several compounds that exhibit highly potent insecticidal activities against SWD by targeting the rdl GABA receptor. Team members in the Zalom Laboratory at UC Davis first detected developing resistance to spinosad in local populations. They have developed isolines of spinosad resistant SWD for genetic studies and have also identified malathion tolerance in a California population of SWD. They have also established colonies of SWD collected from ripe strawberry fruit in the Oxnard, Santa Maria and Salinas/Watsonville areas of California. Obj 3.3: Our project team based at UC Berkeley completed a USDA APHIS Petition to release Ganaspis brasiliensis in North America, provided pupal parasitoids to different laboratories in North America for study, maintained in Quarantine colonies of imported parasitoids, and helped to coordinate classical bio-control activities in North America and between North America and Europe. Greg Loeb, Cornell, found substantial levels of larval parasitism of SWD and D. melanogaster larvae and pupae by the larval parasitoid Ganapsis brasiliensis and G. hookeri, especially in later spring on the island of Hawaii. Phylogenetic analysis of G. brasiliensis by USDA ARS scientist indicates the population from our study is distinct from the race being petitioned for release on the mainland. The Hawaiian race appears to have a broader host range than the race being petitioned for release and there is some speculation it may be a different species. Obj 3.4: The Burrack Laboratory compared the effects of cooler post harvest storage temperatures and longer storage durations than in previous work in blueberries, blackberries, raspberries, and strawberries. The results of this work have been presented at several entomology meetings (see "Products"). We have developed a collaboration with the Economics Team at Cornell University to translate our results into an economic decision aid. Obj 3.5: The Scott Laboratory has begun preliminary laboratory based fitness comparisons of existing genetically sterile lines.

PUBLICATIONS (not previously reported): 2018/09 TO 2019/08
1. Type: Journal Articles Status: Published Year Published: 2019 Citation: Wang, X.-G., Kaçar, G., and Daane, K. M. 2019. Temporal dynamics of host use by Drosophila suzukii in California�s San Joaquin Valley: Implications for area-wide pest management. Insects 10(7), 206. doi.org/10.3390/insects10070206
2. Type: Journal Articles Status: Awaiting Publication Year Published: 2019 Citation: Lee, J. C., Wang., X.-G., Daane, K. M., Hoelmer, K. A., Isaacs, R., Sial, A. A., Walton, V. M. 2019. Biological control of spotted-wing drosophila � current and pending tactics. Journal of Integrated Pest Management 10, 1, (in press) doi.org/10.1093/jipm/pmz012
3. Type: Journal Articles Status: Published Year Published: 2019 Citation: Rodriguez-Saona, C., Vincent, C., and Isaacs, R. 2019. Blueberry IPM: Past successes and future challenges. Annual Review of Entomology 64: 95�114.
4. Type: Journal Articles Status: Published Year Published: 2019 Citation: Rodriguez- Saona, C., Cloonan, K.R., Sanchez-Pedraza, F., Zhou, Y., Giusti, M.M., and Benrey, B. 2019. Differential susceptibility of wild and cultivated blueberries to an invasive frugivorous pest. J. Chem. Ecol. 45: 286�297.
5. Type: Journal Articles Status: Published Year Published: 2019 Citation: Cloonan, K.R., Hernández-Cumplido, J., Viana de Sousa, A.L., Gomes Ramalho, D., Burrack, H.J., Della Rosa, L., Diepenbrock, L.M., Drummond, F.A., Gut, L.J., Hesler, S., Issacs, R., Leach, H., Loeb, G.M., Nielsen, A.L., Nitzsche, P., Park, K.R., Syed, Z., Van Timmeren, S., Wallingford, A.K., Walton, V.M., and Rodriguez-Saona, C. 2019. Laboratory and field evaluation of host-related foraging odor-cue combinations to attract Drosophila suzukii (Diptera: Drosophilidae). J. Economic Entomology. DOI: 10.1093/jee/toz224.
6. Type: Journal Articles Status: Under Review Year Published: 2020 Citation: Urbaneja-Bernat, P., Polk, D., Sanchez-Pedraza, F., Benrey, B., Salamanca, J., and Rodriguez-Saona, C. Non-crop habitats serve as a potential source of spotted-wing drosophila (Diptera: Drosophilidae) to adjacent cultivated highbush blueberries. The Canadian Entomologist. Under Review.
7. Type: Journal Articles Status: Published Year Published: 2019 Citation: Van Timmeren, S., Fanning, P.D., Schöneberg, T., Hamby, K., Lee, J., and Isaacs, R. 2019. Exploring the efficacy and mechanisms of a crop sterilant for reducing infestation by spotted-wing Drosophila (Diptera: Drosophilidae). Journal of Economic Entomology. doi: 10.1093/jee/toz245.
8. Type: Journal Articles Status: Published Year Published: 2018 Citation: Pfab, F., M. V. R. Stacconi, G. Anfora, A. Grassi, V. Walton, and A. Pugliese. 2018. Optimized timing of parasitoid release: a mathematical model for biological control of Drosophila suzukii. Theor Ecol. 1�13.
9. Type: Journal Articles Status: Published Year Published: 2018 Citation: Ioriatti, C., R. Guzzon, G. Anfora, F. Ghidoni, V. Mazzoni, T. R. Villegas, D. T. Dalton, and V. M. Walton. 2018. Drosophila suzukii (Diptera: Drosophilidae) Contributes to the Development of Sour Rot in Grape. J Econ Entomol. 111: 283�292.
10. Type: Journal Articles Status: Published Year Published: 2018 Citation: Grassi, A., A. Gottardello, D. T. Dalton, G. Tait, D. Rendon, C. Ioriatti, D. Gibeaut, R. Stacconi, M. Valerio, and V. M. Walton. 2018. Seasonal Reproductive Biology of Drosophila suzukii (Diptera: Drosophilidae) in Temperate Climates. Environ Entomol. 47: 166�174.
11. Type: Journal Articles Status: Published Year Published: 2018 Citation: Tait G., C. Kaiser, Rossi-Stacconi M. V. , Dalton D.T., Anfora G. Walton V. M. 2018. A food-grade gum as a management tool for Drosophila suzukii. Bulletin of Insectology 71 (2): 295-307
12. Type: Journal Articles Status: Published Year Published: 2019 Citation: Rendon D., Walton V. M. 2019. Drip and overhead sprinkler irrigation in blueberry as cultural control for Drosophila suzukii in Northwestern United States. Economic Entomology J Econ Entomol. 2019 Mar 21;112(2):745-752. doi: 10.1093/jee/toy395.
13. Type: Journal Articles Status: Published Year Published: 2018 Citation: Rendon D., Lee J. C., Tait G., Buser J., Walton V. M. 2018. Survival and fecundity parameters of two Drosophila suzukii morphs on variable diet under suboptimal temperatures. Journal of Insect Science, Volume 18, Issue 6, https://doi.org/10.1093/jisesa/iey113
14. Type: Journal Articles Status: Published Year Published: 2019 Citation: Silva, Cherre Sade Bezerra Da, Price, B.E., Walton, V.M., 2019. Water-Deprived Parasitic Wasps ( Pachycrepoideus vindemmiae ) Kill More Pupae of a Pest ( Drosophila suzukii ) as a Water-Intake Strategy. Scientific Reports 9, 3592. https://doi.org/10.1038/s41598-019-40256-8
15. Type: Journal Articles Status: Published Year Published: 2019 Citation: Silva, Cherre S. Bezerra Da, Price, B.E., Soohoo-Hui, A., Walton, V.M., 2019. Factors affecting the biology of Pachycrepoideus vindemmiae (Hymenoptera: Pteromalidae), a parasitoid of spotted-wing drosophila (Drosophila suzukii). PLOS ONE 14, e0218301. https://doi.org/10.1371/journal.pone.0218301
16. Type: Journal Articles Status: Published Year Published: 2019 Citation: Silva, Cherre Sade Bezerra Da, Park, K.R., Blood, R.A., Walton, V.M., 2019. Intraspecific Competition Affects the Pupation Behavior of Spotted-Wing Drosophila ( Drosophila suzukii ). Scientific Reports 9, 7775. https://doi.org/10.1038/s41598-019-44248-6
17. Type: Journal Articles Status: Published Year Published: 2019 Citation: Rendon, D., Walton, V., Tait, G., Buser, J., Souza, I.L., Wallingford, A., Loeb, G., Lee, J., 2019. Interactions among morphotype, nutrition, and temperature impact fitness of an invasive fly. Ecology and Evolution 9, 2615�2628. https://doi.org/10.1002/ece3.4928
18. Type: Journal Articles Status: Published Year Published: 2019 Citation: Rendon, D., Hamby, K.A., Arsenault?Benoit, A.L., Taylor, C.M., Evans, R.K., Roubos, C.R., Sial, A.A., Rogers, M., Petran, A., Timmeren, S.V., Fanning, P., Isaacs, R., Walton, V.M., 2019. Mulching as a cultural control strategy for Drosophila suzukii in blueberry. Pest Management Science 0. https://doi.org/10.1002/ps.5512
19. Type: Journal Articles Status: Published Year Published: 2019 Citation: Mermer S., Pfab F., Hoheisel G.A., Bahlol H.Y., Khot L., Dalton D.T., Brewer L.J., Rossi Stacconi M.V., Zhang C., Xue L., Walton. V.M. 2019. Canopy spray deposition and related mortality impacts of commonly used insecticides on Drosophila suzukii Matsumura (Diptera: Drosophilidae) populations in blueberry. Pest Management Science. DOI: 10.1002/PS.5672
20. Type: Journal Articles Status: Published Year Published: 2018 Citation: Cloutier, T., J. Collins, and F.A. Drummond. 2018. Octabor (disodium octaborate tetrahydrate) as a Low Toxicity Control Tactic for the Spotted Wing Drosophila, Drosophila suzukii (Matsumura). J. Agric. Studies 6(3): 63-75.
21. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Drummond, F.A., Collins, J.A., Al-Najjar, G. and Christensen, J. 2019. Itty-bitty traps for monitoring spotted wing Drosophila (Drosophila suzukii Matsumura), does size matter? In Proceedings of the North American Blueberry Research and Extension Workers Conference. August 12-15, 2018, Orono, ME. 9 pp. University of Maine Digital Commons: https://digitalcommons.library.umaine.edu/cgi/viewcontent.cgi?article=1075&cont ext=nabrew2018
22. Type: Journal Articles Status: Published Year Published: 2019 Citation: Drummond, F.A., E. Ballman, and J. A. Collins. 2019. Spotted wing drosophila (Drosophila suzukii (Matsumura)) adult movement, activity, and oviposition behavior in Maine wild blueberry (Vaccinium angustifolium Aiton). Journal of Economic Entomology. https://doi.org/10.1093/jee/toz059
23. Type: Journal Articles Status: Published Year Published: 2019 Citation: Drummond, F.A., J. Collins, and E. Ballman. 2019. Population dynamics of spotted wing drosophila (Drosophila suzukii (Matsumura)) in Maine wild blueberry. Insects 10(7): 205-229. https://doi.org/10.3390/insects10070205
24. Type: Journal Articles Status: Published Year Published: 2019 Citation: Ballman, E. and F.A. Drummond. 2019. Larval movement of spotted wing drosophila, Drosophila suzukii (Diptera: Drosophilidae). J. Kans. Entomol. Soc. 92(1): 412-421.
25. Type: Journal Articles Status: Published Year Published: 2019 Citation: Drummond, F.A. In Review. Early spring buildup of an invasive insect fruit pest, the potential role of fungal fruiting bodies. Journal of Bacteriology & Mycology. 6(5): 113. https://austinpublishinggroup.com/bacteriology/fulltext/bacteriology-v6-id1113. pdf
26. Type: Journal Articles Status: Published Year Published: 2019 Citation: Rendon, D., Walton, V., Tait, G., Buser, J., Lemos Souza, I., Wallingford, A. Loeb, G., and Lee, J. 2019. Interactions among morphotype, nutrition, and temperature impact fitness of an invasive fly. Ecology and Evolution. DOI:10.1002/ece3.4928
27. Type: Journal Articles Status: Published Year Published: 2019 Citation: Stockton, D., Brown, R., and Loeb, G. 2019. Not very hungry? Discovering the hidden food sources of a small fruit specialist, Drosophila suzukii. Ecological Entomology, DOI: 10.1111/een.12766.
28. Type: Journal Articles Status: Published Year Published: 2019 Citation: Fanning, P., Luttinen, B.E., Johnson, A.E., Espeland, E.M., Jahn, N.T., and Isaacs, R. 2019. Behavioral and physiological resistance to desiccation in spotted wing Drosophila, Drosophila suzukii. Environmental Entomology 48, 792-798.
29. Type: Journal Articles Status: Published Year Published: 2019 Citation: Leach, H., Van Timmeren, S., Wetzel, W. and Isaacs, R. 2019. Predicting within- and between-year variation in populations of the invasive spotted wing Drosophila (Diptera: Drosophilidae) in a temperate region. Environmental Entomology. doi: 10.1093/ee/nvz101
30. Type: Journal Articles Status: Published Year Published: 2019 Citation: Leach, H., Stone, J., Van Timmeren, S., and Isaacs, R. 2019. Stage-specific and seasonal induction of the overwintering morph of spotted wing Drosophila (Diptera: Drosophilidae). Journal of Insect Science 19, 5. https://doi.org/10.1093/jisesa/iez067
31. Type: Journal Articles Status: Published Year Published: 2019 Citation: Rendon, D., Hamby, K.A., Arsenault?Benoit, A.L., Taylor, C.M., Evans, R.K., Roubos, C.R., Sial, A.A., Rogers, M., Petran, A., Van Timmeren, S., Fanning, P., Isaacs, R., and Walton, V. 2019. Mulching as a cultural control strategy for Drosophila suzukii in blueberry. Pest Management Science doi.org/10.1002/ps.5512
32. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Sial, A., R. Isaacs, M. Grieshop.2, C. Guédot, K. Hamby, V. Walton, M. Rogers, O. Liburd, D. Johnson, F. Zalom, H.J. Burrack, J. Lee, and T.C. Leskey. 19 November 2019. OREI Update: Development and implementation of systems based organic management strategies for spotted-wing drosophila. Entomological Society of America Annual Meeting. St. Louis, MO.
33. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Elsensohn, J., A. Kokotovich, J. Delborne, H.J. Burrack. 17 November 2019. Lessons from using engagement to elicit potential impacts of emerging technologies in invasive pest management: Drosophila suzukii as a case study. Entomological Society of America Annual Meeting. St. Louis, MO.
34. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Burrack, H.J., R. Isaacs, C. Rodriguez-Saona, A. Sial, V. Walton, M. Scott, Z. Brown, L. Gut, K. Dong, F. Drummond, N. Wiman, M. Gomez, J. Chiu, F. Zalom, K. Daane, K. Hoelmer, Z. Syed. 19 November 2019. SCRI update: Developing and implementing sustainable strategies to manage spotted-wing drosophila in United States fruit crops. Entomological Society of America Annual Meeting. St. Louis, MO.
35. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Kraft, L. and H.J. Burrack. 12 November 2018. Determining the effect of postharvest cold-storage treatment on the survival of immature Drosophila suzukii in small fruits. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting. Vancouver, BC. Poster presentation.
36. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Kraft, L., L. Diepenbrock, T. Sit, H.J. Burrack. 18 November 2019. A new tool in the toolbox? Using gut molecular content to monitor spotted-wing drosophila movement. Entomological Society of America Annual Meeting. St. Louis, MO. Poster presentation.
37. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Sial, A., R. Isaacs, F. Zalom, B. Gress, P. Fanning, S. Van Timmeren, N. Spaulding, J. Disi, O. Liburd, F.A. Drummond, K. Hamby, C. Rodriguez-Saona, N. Sarkar, R. Holdcraft, M. Lewis, J.A. Collins, L. Diepenbrock, and HJ. Burrack. 12 November 2018. Spotted-wing drosophila � An invasive pest that has changed blueberry insect management and export considerations. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting. Vancouver, BC.
38. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Burrack, H.J. R. Isaacs, C. Rodriguez-Saona, A. Sial, V. Walton, M. Scott, Z. Brown, L. Gut, K. Dong, F. Drummond, N. Wiman, M. Gomez, J. Chiu, F. Zalom, K. Daane, K. Hoelmer, Z. Syed. 13 November 2018. SCRI update: Developing and implementing sustainable strategies to manage spotted-wing drosophila in United States fruit crops. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting. Vancouver, BC. Recorded and presented remotely.
39. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Isaacs, R., A. Sial, F. Zalom, B. Gress, S. Van Timmeren, N. Spaulding, J. Disi, O. Liburd, F.A. Drummond, K. Hamby, C. Rodriguez, L. Diepenbrock, H.J. Burrack and N. Sarkar. 13 November 2018. An update on insecticide resistance monitoring for SWD in berry crops. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting. Vancouver, BC.
40. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Sial, A., R. Isaacs, M. Grieshop.2, C. Guédot, K. Hamby, V. Walton, M. Rogers, O. Liburd, D. Johnson, F. Zalom, H.J. Burrack, J. Lee, and T.C. Leskey. 13 November 2018. OREI Update: Development and implementation of systems based organic management strategies for spotted-wing drosophila. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting. Vancouver, BC.
41. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Burrack, H.J., J. Elsensohn, J. Delborne, and A. Kokovich. 14 November 2018. Assessing risks of emerging technologies in pest management through expert elicitation. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting. Vancouver, BC. Recorded and presented remotely.
42. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Kraft, L., M. Scott, and H.J. Burrack. 14 November 2018. How fit are female-lethal flies? Determining if genetically modified berry pests are ready for the field. Entomological Society of America, Entomological Society of Canada, and Entomological Society of British Columbia Joint Annual Meeting. Vancouver, BC.
43. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Sial, A. A., Developing sustainable IPM programs for spotted-wing drosophila in organic systems: A continuing battle. The 93rd Annual Meeting of Southeastern Branch of ESA, 3-6 March 2019, Mobile, AL.
44. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Dong, K. Aug. 2019 Symposium in the 2019 ACS International Award for Research in Agrochemicals Symposium honoring Dr. Vince Salgado (BASF), San Diego, CA.
45. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Zalom, F.G., 2018. Status of Drosophila Suzuki in the United States. XVII Brazilian Congress of Entomology/X Latin-American Congress of Entomology, September 2-6, 2018, Gramado, Brazil
46. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: D. Adeline Yeh*, Miguel I. Gómez, C.-Y. Cynthia Lin Lawell and Xiaoli Fan. �A Farm-level Bioeconomic Model of Invasive Species Management: The Case of Spotted Wing Drosophila in Maine�. Conference presentation at the 2019 Agricultural and Applied Economics Association Annual Meeting.
47. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Gress, B.E. and F.G. Zalom. 2018. Identification and risk assessment of spinosad resistance in a California population of Drosophila suzukii. Entomological Society of America Annual Meeting, November 11, 2018, Vancouver BC.
48. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Disi J. and A. Sial 2018. RAPID Screening of Residual Toxicity of Insecticides Against Spotted Wing Drosophila (Diptera: Drosophilidae) in Treated Vials Over Time. 2018 Joint Meeting of the Georgia and South Carolina Entomological Societies. Unicoi State Park and Lodge Helen, Georgia. 4 � 6 April, 2018.
49. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Hunter J. and A. Sial 2018. Lethal effects of selected insecticides on the immature life stages of Drosophila suzukii (Matsumura). 2018 Joint Meeting of the Georgia and South Carolina Entomological Societies. Unicoi State Park and Lodge Helen, Georgia. 4 � 6 April, 2018.
50. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Spaulding R. N and A. A. Sial (2018). Screening for insecticide resistance in spotted wing drosophila in Georgia. 2018 Joint Meeting of the Georgia and South Carolina Entomological Societies. Unicoi State Park and Lodge Helen, Georgia. 4 � 6 April, 2018.
51. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Sial A. (2018). An integrated approach to managing spotted wing drosophila (Diptera: Drosophilidae).2018 Joint Meeting of the Georgia and South Carolina Entomological Societies. Unicoi State Park and Lodge Helen, Georgia. 4 � 6 April, 2018.
52. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Spaulding R. N and A. Sial 2018. Monitoring for insecticide resistance in spotted wing drosophila (Drosophilia suzukii) in Georgia. ESA, ESC and ESBC Joint Annual Meeting, Vancouver Canada. 11-14 November 2018
53. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Hunter J. and A. Sial 2018. Impact of insecticide treatments on immature life stages of Drosophila suzukii (Matsumura). ESA, ESC and ESBC Joint Annual Meeting, Vancouver Canada. 11-14 November 2018
54. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Disi J. and A. Sial 2019. Little changes big impacts - a comparison between CDC and RAPID bottle bioassays for testing insecticide activities. 93rd Annual meeting of The Southeastern Branch Entomological Society of America Mobile, Alabama. March 3�6, 2019.
55. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Sial A., C. Roubos, J. Disi, J. Hunter 2019. Assessment of new products and formulations for organic management of spotted-wing drosophila. Entomological Society of America Annual Meeting, St Louis, MO 17-20 Nov. 2019
56. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Nieri, R., HC Cromwell, V. Walton, MV Rossi Stacconi and N. Wiman. 2019. Behavioral manipulation of Drosophila suzukii exposed to different oviposition substrates., ESA Pacific Branch Meeting, San Diego, California (USA).
57. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Nieri, R., MV. Rossi Stacconi, N. Wiman and V. Walton. 2018. Behavioral manipulation of Drosophila suzukii: immediate and longterm effects of different oviposition substrates exposure. ESA, ESC and ESBC Joint Annual Meeting, Vancouver (Canada)
58. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Syed, Z. 2019. Insights from genomes, transcriptomes and proteomes for insect manipulations. Symposium �Linking Genes to Behavior: Expanding the Targets for Behaviorally-Based Management of Insects�. Hosts: Erin Scully and Rob Morrison. ESA North Central meet. Cincinnati, OH
59. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Wang, XG et al. 2018. Progress and challenges of biological control of spotted-wing drosophila using parasitoids. Entomological Society of American & Entomological Society of Canada Annual Meeting. Vancouver, Canada. Nov 2018
60. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Rodriguez-Saona, C., Urbaneja-Bernat, P., Salazar-Mendoza, P., Cloonan, K., and Zhang, A. 2019. Scents from the wild: The vinegar fly Drosophila suzukii prefers volatiles from wild than cultivated blueberries. The 10th Conference of Asia-Pacific Association of Chemical Ecologists (APACE). Hangzhou, China.
61. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Paolo Salazar-Mendoza, Rodriguez-Saona, C., Urbaneja-Bernat, P., Cloonan K., Zhang, A. 2019. Wild attraction: responses of the vinegar fly Drosophila suzukii to volatiles from wild and cultivated blueberries. II International Workshop on Chemical Ecology of Multitrophic Interactions. Lavras, Brazil.
62. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Urbaneja-Bernat, P. and Rodriguez-Saona, C. 2019. Wild blueberries are more attractive than cultivated blueberries to the invasive vinegar fly Drosophila suzukii. 35th Annual Meeting of the International Society of Chemical Ecology. Atlanta, Georgia.
63. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Rodriguez-Saona, C. and Cloonan, K. 2018. Double-edged sword: Cultivated blueberries are more susceptible, but less attractive, to spotted wing drosophila than wild blueberries. 94th Cumberland�Shenandoah Fruit Workers Conference. Winchester, Virginia.
64. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Cloonan, K. and Rodriguez-Saona, C. 2018. Domestication reduces the attraction of a vinegar fly to fruit volatiles. Entomological Society of America Annual Meeting. Vancouver, Canada.
65. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Cloonan, K. and Rodriguez-Saona, C. 2018. Red-sticky traps: a more friendly method for capturing the spotted wing drosophila. Entomological Society of America Annual Meeting. Vancouver, Canada.
66. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Loeb, G., Hesler, S, Riggs, D., Lesky, T, McDermott, L, and Pritts, M. 2018. Excluding Drosophila suzukii from NY raspberries and blueberries using netting. A contribution as part of a symposium organized by W. Hamilton titled �Multistate research collaborations accelerate solutions to spotted wing drosophila Drosophila suzukii management�, at the 2018 ESA annual meeting held in Vancouver, Canada.
67. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Stockton, D.G., and Loeb, G. M. 2018. Overwintering biology of SWD: acclimation and survival outcomes. Invited speaking presentation at the annual Entomological Society of America meeting, group meeting WERA 1021; Vancouver, British Columbia, Canada.
68. Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Stockton, D., Hesler, S, and Loeb, G. 2018. Factors affecting overwintering outcomes in drosophila suzukii. Contributed presentation at the ESA Eastern Branch meeting in Annapolis, MD.
69. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Walton V.M., Tait, G.; Rossi Stacconi, M.V.; Dalton, D.; and Anfora, G. 2019. A food-grade gum as a management tool for Drosophila suzukii. In: 92nd Annual Orchard Pest and Disease Management Conference, Portland, OR, 8-10 January, 2019.
70. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: A. Abrieux, K.M. Lewald, J.C. Chiu,. The molecular and neuronal basis of the insect photoperiodic timer. European Biological Rhythms Society Congress, Lyons, France, August 25-29, 2019.
71. Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Chiu, J.C. 2019. The molecular and neuronal basis of the insect photoperiodic timer, Chronobiology Gordon Research Conference, Castelldefels, ES, Spain, June 23, 2019.
72. Type: Other Status: Published Year Published: 2019 Citation: Wiman, NG, J Pscheidt, and E Peachey. 2019. Cherry pest management guide for the Willamette Valley. Oregon State University Extension Service Publication EM 8329.
73. Type: Other Status: Published Year Published: 2019 Citation: Rodriguez-Saona, C., D. Polk, and K. Cloonan. 2019. Using red sticky traps for spotted wing drosophila. Proceedings of the Atlantic Coast Agricultural Convention and Trade Show. Atlantic City, New Jersey.
74. Type: Other Status: Published Year Published: 2019 Citation: Rodriguez-Saona, C, C. Michel, and N. Firbas. 2019. Efficacy of traps for monitoring spotted wing drosophila. Proceedings of the Atlantic Coast Agricultural Convention and Trade Show. Atlantic City, New Jersey.
75. Type: Other Status: Published Year Published: 2019 Citation: Rodriguez-Saona, C., D. Polk, and K. Cloonan 2019. Trapping for SWD vs. Infestation in Blueberries. Proceedings of the Mid-Atlantic Fruit & Vegetable Convention. Hershey, PA.
76. Type: Other Status: Published Year Published: 2019 Citation: Wilson, J., R. Isaacs and L. Gut. 2019. Michigan spotted wing Drosophila update � June 26, 2019. MSU AgNews, June 26, 2019.
77. Type: Other Status: Published Year Published: 2019 Citation: Wilson, J., R. Isaacs and L. Gut. 2019. Michigan spotted wing Drosophila update � July 5,2019. MSU AgNews, July 5, 2019.
78. Type: Other Status: Published Year Published: 2019 Citation: Wilson, J., R. Isaacs and L. Gut. 2019. Michigan spotted wing Drosophila update � July 9,2019. MSU AgNews, July 9, 2019.
79. Type: Other Status: Published Year Published: 2019 Citation: Wilson, J., R. Isaacs and L. Gut. 2019. Michigan spotted wing Drosophila update � July 16,2019. MSU AgNews, July 16, 2019.
80. Type: Other Status: Published Year Published: 2019 Citation: Wilson, J., R. Isaacs and L. Gut. 2019. Michigan spotted wing Drosophila update � July 24,2019. MSU AgNews, July 24, 2019.
81. Type: Other Status: Published Year Published: 2019 Citation: Wilson, J., R. Isaacs and L. Gut. 2019. Michigan spotted wing Drosophila update � July 30,2019. MSU AgNews, July 30, 2019
82. Type: Other Status: Published Year Published: 2019 Citation: Sial, A. A. 2019. Spotted Wing Drosophila: Identification, Monitoring and Management in Georgia Blueberries. Dixie-Blueberry News, Georgia Blueberry Growers Association Newsletter, 19(3): 6-12.
83. Type: Other Status: Published Year Published: 2019 Citation: Sial, A. A. 2019. UGA Suggested Season-long Insecticide Rotational Programs for Spotted Wing Drosophila Control in Blueberries. Dixie-Blueberry News, GA Blueberry Growers Association Newsletter, 19(3): 5.
84. Type: Other Status: Published Year Published: 2019 Citation: Sial, A. A. 2019. UGA Suggested Insecticide Regime for Spotted Wing Drosophila Control in Blueberries. Dixie-Blueberry News, Georgia Blueberry Growers Association Newsletter, 19(3): 33-34.
85. Type: Other Status: Published Year Published: 2019 Citation: Sial, A. A. 2019. Grower Handouts for � 1) Identification of Spotted Wing Drosophila; 2) Spotted Wing Drosophila Lifecycle; 3) Management of Spotted Wing Drosophila in Blueberries; and 4) Monitoring Spotted Wing Drosophila Using Traps. Dixie-Blueberry News, Georgia Blueberry Growers Association Newsletter, 19(3): 35-38.
86. Type: Other Status: Published Year Published: 2019 Citation: Yeh D.A., F.A. Drummond, and M.I. Gómez. 2019. �Economic Management Support Tool for Spotted Wing Drosophila (SWD) in Maine Wild Blueberry Production� University of Maine Cooperative Extension.
87. Type: Other Status: Published Year Published: 2019 Citation: Drummond, F.A., J.A. Collins, and E. Ballman. 2019. Insect pest management experiments on wild blueberries in Maine, 2018. Maine Blueberry Commission, 101 pp.
88. Type: Other Status: Published Year Published: 2019 Citation: Drummond, F.A., J. Collins, and E. Ballman. 2018. Spotted wing Drosophila: Pest biology and IPM recommendations for wild blueberries. Univ. Maine Coop. Ext. Fact Sheet 210, 6 pp. https://extension.umaine.edu/blueberries/factsheets/insects/210-spotted-wing-dr osophila/ Last accessed 78 March. 2019.
89. Type: Other Status: Published Year Published: 2019 Citation: Yarborough, D., F.A. Drummond, and J.A. Collins. 2019. Insect control guide for wild blueberries. Univ. Maine Coop. Ext. Fact Sheet No. 209, UMaine Extension No. 2001, 14 pp.
90. Type: Other Status: Published Year Published: 2019 Citation: Stockton, D.G., Hesler, S. P., McDermott, L., Riggs, D.I., & Loeb, G.M. 2019. The efficacy of exclusion netting for Spotted-wing drosophila control in New York State. New York State Berry Growers Association Newsletter, March 13, 2019.
91. Type: Other Status: Published Year Published: 2019 Citation: Stockton, D.G., & Loeb, G.M. 2019. There�s snow place like home: Investigating the behavior and biology of locally overwintered Spotted-wing drosophila. Fruit Quarterly. Winter Issue, Dec 2019.
92. Type: Other Status: Published Year Published: 2019 Citation: Drummond, F.A., and J.A. Collins. In Press. Insect pest management experiments on wild blueberries in Maine, 2019. Maine Blueberry Commission, 10 pp.
93. Type: Journal Articles Status: Awaiting Publication Year Published: 2019 Citation: Kokotovich, A.E., J. Delborne, J. Elsensohn, H. Burrack. In press. Emerging technologies for invasive insects: the role of engagement. Annals of the Entomological Society of America.
94. Type: Journal Articles Status: Published Year Published: 2019 Citation: Stockton, D.G., A. Wallingford, D. Rendon, P. Fanning, C.K. Green, L. Diepenbrock, E. Ballman, V.M. Walton, R. Isaacs, H. Leach, A.A. Sial, F. Drummond, H. Burrack, and G.M Loeb. 2019. Interactions between biotic and abiotic factors affect survival in overwintering Drosophila suzukii (Matsumura). Environmental Entomology. 48(2): 454-464.
95. Type: Journal Articles Status: Published Year Published: 2018 Citation: Diepenbrock, L.M., T. Sit, J. Lundgren, and H.J. Burrack. 2018. Detecting specific resource use by Drosophila suzukii (Diptera: Drosophilidae) using gut content analysis. Journal of Economic Entomology. 111(3): 1496-1500.
96. Type: Journal Articles Status: Published Year Published: 2019 Citation: Green, C. K., P. J. Moore, and A. A. Sial. 2019. Impact of heat stress on development and fertility of Drosophila suzukii Matsumura (Diptera: Drosophilidae). J. Insect Physiol. 114, 45-52. doi: 10.1016/j.jinsphys.2019.02.008
97. Type: Journal Articles Status: Published Year Published: 2019 Citation: Van Timmeren, S., A. A. Sial, S. Lanka, N. Spaulding, and R. Isaacs. 2019. Development of a rapid assessment method for detecting insecticide resistance in spotted-wing Drosophila (Drosophila suzukii). Pest Manag. Sci. 75(7): 1782-1793. doi: 10.1002/ps.5341
98. Type: Journal Articles Status: Published Year Published: 2019 Citation: Lee, J. C., X. Wang, K. M. Daane, K. A. Koelmer, R. Isaacs, A. A. Sial, and V. M. Walton. 2019. Biological control of spotted-wing drosophila (Diptera: Drosophilidae) � current and pending tactics. J. Integr. Pest Manag. 10(1): 1-9. doi: 10.1093/jipm/pmz012
99. Type: Journal Articles Status: Awaiting Publication Year Published: 2019 Citation: Swoboda-Bhattaraic, K.A. and H.J. Burrack. In press. Diurnal and Seasonal Activity Patterns of Drosophilid Species (Diptera: Drosophilidae) Present in Blackberry Agroecosystems with a Focus on Spotted-Wing Drosophila. Environmental Entomology.
100. Type: Journal Articles Status: Published Year Published: 2019 Citation: Schmidt, J. M., T. S. Whitehouse; C. K. Green; H. Krehenwinkel; R. Schmidt-Jeffris, and A. A. Sial. 2019. Local and landscape-scale heterogeneity shape spotted-wing drosophila (Drosophila suzukii) activity and natural enemy abundance: implications for trophic interactions. Agric. Ecosyst. Environ., 272: 86-94. doi: 10.1016/j.agee.2018.11.014
101. Type: Other Status: Published Year Published: 2019 Citation: Hunter, J. H. and A. A. Sial. 2019. Efficacy of Improved Management Program in Comparison with Grower Standard Program to Control Drosophila suzukii in Rabbiteye Blueberry, 2017. Arthropod Management Tests, 44(1). doi:10.1093/amt/tsz065
102. Type: Other Status: Published Year Published: 2019 Citation: Hunter, J. H. and A. A. Sial. 2019. Efficacy of Improved Management Program in Comparison with Grower Standard Program to Control Drosophila suzukii in Rabbiteye Blueberry, 2018. Arthropod Management Tests, 44(1). doi:10.1093/amt/tsz066
103. Type: Other Status: Published Year Published: 2019 Citation: Disi, J. O. and A. A. Sial. 2019. Efficacy of HOOK SWD attract-and-kill SPLAT for management of spotted-wing drosophila in Georgia rabbiteye blueberries. Arthropod Management Tests, 2019. 44 (C13).
104. Type: Journal Articles Status: Awaiting Publication Year Published: 2020 Citation: Xiaoli Fan, Miguel I. Gómez, Shadi S. Atallah and Jon Conrad. �A Bayesian State-Space Approach for Invasive Species Management: The Case of Spotted Wing Drosophila.� American Journal of Agricultural Economics. Forthcoming.
105. Type: Journal Articles Status: Awaiting Publication Year Published: 2020 Citation: D. Adeline Yeh, Francis A. Drummond, Miguel I. Gómez, and Xiaoli Fan. �The Economic Impacts and Management of Spotted Wing Drosophila (Drosophila Suzukii): The Case of Wild Blueberries in Maine" Journal of Economic Entomology.
106. Type: Journal Articles Status: Published Year Published: 2019 Citation: Gress, B.E. and F.G. Zalom. 2019. Identification and risk assessment of spinosad resistance in a California population of Drosophila suzukii. Pest Manag. Sci. 75: 1270-1276. https://doi.org/10.1002/ps.5240
107. Type: Journal Articles Status: Published Year Published: 2018 Citation: Wang, X.�G., Nance, A., Jones, J. M. L., Hoelmer, K. A., and Daane, K. M. 2018. Aspects of the biology and developmental strategy of two Asian larval parasitoids evaluated for classical biological control of Drosophila suzukii. Biological Control 121: 58-65. doi.org/10.1016/j.biocontrol.2018.02.010
108. Type: Journal Articles Status: Published Year Published: 2018 Citation: Wang, X.�G., Serrato, M. A., Son, Y., Walton, V. M., and Daane, K. M. 2018. Thermal performance of two indigenous pupal parasitoids attacking the invasive Drosophila suzukii (Diptera: Drosophilidae). Environmental Entomology 47(3):764-772. doi: 10.1093/ee/nvy053
109. Type: Journal Articles Status: Published Year Published: 2019 Citation: Giorgini, M., Wang, X.-G., Wang, Y., Chen, F.-U., Hougardy, E., Hong-Mei, Zhang, H.-M., Chen, Z.-Q., Chen, H.-Y., Liu, C.-X., Casconea, P., Formisano, G. Carvalho, G. A., Biondi, A., Buffington, M., Daane, K. M., Hoelmer, K. A., and Guerrieri, E. 2019. Exploration for native parasitoids of Drosophila suzukii in China reveals a diversity of parasitoid species and narrow host range of the dominant parasitoid Journal of Pest Science. https://doi.org/10.1007/s10340-018-01068-3
110. Type: Journal Articles Status: Published Year Published: 2019 Citation: Wang, X-G., Hougardy, E., Nance, A. H., Hogg, B. N., Hoelmer, K. A., and Daane, K. M. 2019. Potential competitive outcomes among three solitary larval endoparasitoids as candidate agents for classical biological control of Drosophila suzukii. Biological Control 130: 18-26. https://doi.org/10.1016/j.biocontrol.2018.12.003
111. Type: Journal Articles Status: Published Year Published: 2019 Citation: Hougardy, E., Hogg, B. N., Wang, X.-G., and Daane, K. M. 2019 Comparison of thermal performances of two Asian larval parasitoids of Drosophila suzukii. Biological Control 136: https://doi.org/10.1016/j.biocontrol.2019.104000

PROGRESS: 2016/09/01 TO 2017/08/31
Target Audience:The target audiences of this project are growers of crops impacted by spotted wing drosophila (SWD) and the extension personnel that serve them. Our primary efforts are directed at US cherry, blueberry, blackberry, raspberry, and strawberry growers, but we also engage with growers of other crops with SWD concerns. Our project team has also developed international connections to facilitate information transfer to other parts of the world where SWD is a significant pest. Efforts during the past year have included a total of 50 extension online and print publications, see "Products" for details. The project teamhas led or participated in a total of 62in-person extension meetings or workshops that have reached at least 2181 members of our target audience. These workshops, along with attendance information when available, are listed below.We also presented the first of four planned webinars on 25 January 2017 to a total 272 live attendees. This webinar is available online and has been viewed an additional 851 times as of this report,https://www.youtube.com/watch?v=nV4Yb6 DiHw. 1. Ballman, E. and F.A. Drummond. 2017. Maine Agricultural Trade Show, Augusta, ME. 2. Burrack, H.J. Georgia Strawberry School. 9 August 2017. Presented remotely.14 attendees. 3. Burrack, H.J. Virginia Strawberry School. 28 February 2017. Virginia Beach, VA. 85 attendees. 4. Burrack, H.J. Southeastern Fruit & Vegetable Conference Caneberry Session. 6 January 2017. Savannah, GA. 75 attendees. 5. Burrack, H.J. Bladen County Blueberry Meeting. 13 February 2016. Elizabethtown, NC. 45 attendees. 6. Burrack, H.J. Blueberry Open House. 11 January 2017. Fayetteville, NC. 53 attendees. 7. Burrack, H.J. Great Lakes Fruit & Vegetable Expo Blueberry Session. 7 December 2016. Grand Rapids, MI. 90 attendees. 8. Burrack, H.J. Great Lakes Fruit & Vegetable Conference Cherry Sessoion. 7 December. Grand Rapids, MI. 65 attendees. 9. Burrack, H.J. Great Lakes Fruit & Vegetable Expo and North American Raspberry and Blackberry Association. 6 December 2016.Grand Rapids, MI. 85 attendees. 10. Daane KM, Wang XG. California Cherry Research Review. Stockton, CA, Jan. 2017 11. Daane KM, Wang XG. Sweet Cherry Workgroup Meeting. Stockton, CA, Oct. 2016. 12. Diepenbrock, L.M., Swoboda-Bhattarai, K.A., Johnson, D.T., and H.J. Burrack. NC Blackberry and Raspberry Growers Association Meeting. Shelby, NC. 35 attendees. 13. Diepenbrock, L.M. and H.J. Burrack. Blueberry Education Workshop, Hattiesburg, MS. 20 attendees. 14. Diepenbrock, L.M., Hamby, K., Walton, V, and H.J. Burrack. Southeast Regional Fruit and Vegetable ConferenceCaneberry Session. Savannah, GA, 2017. 75 attendees. 15. Drummond, F.A. 2017. New Brunswick Wild Blueberry Annual Meeting. St. Andrews, NB, Canada 16. Drummond, F.A. 2017. Maine Blueberry School, Ellsworth, ME. March 2017. 17. Drummond, F.A. 2017. Maine Blueberry School, Waldoboro, ME. March 2017. 18. Drummond, F.A. 2017. Maine Blueberry School, Machias, ME. March 2017. 19. Drummond, F. 2017. Annual Summer Wild Blueberry Field Day, Jonesboro, ME. 20. Fanning, P. and R. Isaacs. SWD workshop, Trevor Nichols Research Center, Fennville, MI. 26 people. 21. Fanning, P., S. Van Timmeren, S., and R. Isaacs. Mar 2017. Wilbur Ellis Information Seminar, Dundee MI. 35 growers. 22. Fanning, P., S. Van Timmeren, J. Wise, J., and R. Isaacs. Sept. 2016. SWD Summit, NWMREC, Traverse City, MI. 38 growers. 23. Gut, L. Mar 2017. Southeast MI Spring Tree Fruit Meeting. Flint, MI. 24. Gut, L. Mar 2016. Southeast MI Spring Tree Fruit Meeting. Flint, MI. 25. Gut, L. Feb 2017. Southwest Michigan Horticultural Days, Benton Harbor, MI. 26. Gut, L. Jan 2017. Crop Production Services Specialty Crop Meeting. Mt. Pleasant, MI. 27. Gut, L. Jan 2017. Wisconsin Fresh Fruit & Vegetable Conference. Waterloo, WI. 28. Gut, L. Jan 2017. Northwest Michigan Horticultural Show, Acme, MI. 29 to 30. Gut, L. Dec 2016. Michigan State Horticultural Society. Grand Rapids, MI. 31. Gut, L. Dec 2016. DuPont Crop Protection VIP Knowledge Transfer. Grand Rapids, MI. 32. Gut, L. Nov 2016. SWD Summit. Northwest Michigan Horticultural Research Center. 33. Hesler, S., Loeb, G., Cha, D., Jentsch, P., Zamen, F., Carroll, J., and Nyrop, P. Cornell Fruit Field Day in Geneva, NY. 20 July 2016. Approximately 50 attendees. 34.Isaacs, R. Pre-harvest blueberry extension meeting. Trevor Nichols Research Center. June 30, 2017. 56 growers. 35. Isaacs, R. Michigan State Horticultural Society Board Meeting. Clarksville Research Center, Clarksville, MI. June 7, 2017. 6 growers. 36. Isaacs, R. Parallel 45 Spring Kickoff Meeting. Northwest Michigan Horticultural Research Center, Traverse City, MI. May 5, 2017. 35 people. 37. Isaacs, R. Blueberry insect pest management update. Haven Harvesters, South Haven, MI. April 27, 2017. 55 people. 38. Isaacs, R. Insect Management Update. Fruit Hill growers. Paw Paw, Michigan. April 13, 2017. 36 growers. 39. Isaacs, R. Insecticide update and late-season pest survey. Southwest Hort Days, Benton Harbor, MI. February 2, 2017. 65 people. 40. Isaacs, R. Blueberry insect update. Southwest Hort Days, Benton Harbor, MI. February 2, 2017. 42 people. 41. Isaacs, R. Great Lakes Expo Caneberry Session, Grand Rapids, MI. December 6, 2016. 250 people. 42. Isaacs, R. Great Lakes Expo Strawberry Session, Grand Rapids, MI. December 6, 2016. 70 people. 43. Isaacs, R. Exclusion netting and harvest frequency for SWD management. Great Lakes Expo, Grand Rapids, MI. December 5, 2016. 80 people. 44. Isaacs, R., P. Fanning, S. Van Timmeren, A. Van Woerkom and J. Wise. DuPont Information Seminar. Great Lakes Expo, Grand Rapids, MI. 5 December2016. 45. Isaacs, R. Sept. 2016. Fruit Team Summer Tour. First Pick Farms, Rothbury, MI. 1 September2016. 46. Leach, H. and R. Isaacs. Poster Presentation at Great Lakes Expo, Grand Rapids, MI. December 5-8, 2016. (poster).Mar 2017. 47. Loeb, G. and Hesler, S. 2017. A closer look at lures and exclusion netting: Interactive demonstration. Empire State Producers Expo in Syracuse, NY. Approximately 75 attendees. 48 to 49. Loeb, G. 2016. Two sessions in Spotted Wing Drosophila Summit at the Northwest Michigan Horticulture Research Center, Traverse City, MI on 9 November 2016. Presented remotely.Approximately 30 attendees. 50. Rodriguez-Saona, C. 2017. Spotted wing drosophila: A research update. Atlantic Coast Agricultural Convention and Trade Show. Atlantic City, New Jersey. 51. Sial, A. A. and H. J. Burrack. Southeastern Regional Fruit and Vegetable Conference Blueberry Session, Savannah International Trade & Convention Center, 6-9 Jan 2017, Savannah, GA. 52. Sial, A. A. Southeastern Regional Fruit and Vegetable Conference Muscadine Session, Savannah International Trade & Convention Center, 6-9 Jan 2017, Savannah, GA. 53. GA. Grant, J. A. and Sial, A. A. Southeastern Regional Fruit and Vegetable Conference Blueberry Session, Savannah International Trade & Convention Center, 6-9 Jan 2017, Savannah, GA. 54. Evans, R. K. and Sial, A. A. Southeastern Regional Fruit and Vegetable Conference Blueberry Session, Savannah International Trade & Convention Center, 6-9 Jan 2017, Savannah, GA. 55. Sial, A. A. Georgia Blueberry Growers Meeting - Annual Blueberry Update, 4 Jan 2017 Alma, GA. 56. Sial, A. A. 27 October 2016, Brantley, Clinch, and Ware Counties Blueberry Meeting, Manor, GA. 57. Wallingford, A. and Loeb, G. 2016. Cornell Fruit Field Day in Geneva, NY. 20 July 2016. Approximately 50 attendees. 58. Walton, V. Blueberry Field day, NWREC, Aurora, July 6, 2016. 100 attendees. 59. Walton, V. Blueberry field Day, Aurora, July 8, 2016. 100 attendees. 60. Walton, V. Wasco County cherry breakfast growers meeting, The Dalles, Oregon, April 29, 2016. 60 attendees. 61. Walton, V. North Willamette Horticultural Society, January 15, 2017. 300 attendees. 62. Walton, V. Blueberry Commission Research update. January 9 2017. 50 attendees. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has directly contributed to the training of 11 post docs, 6 graduate students, and 16 undergraduates. In addition, we have developed and participated in extensive extension training sessions and scientific meetings. How have the results been disseminated to communities of interest?See "Target Audience" for detailed information on stakeholder extension and outreach. In addition to these activities we have made numerous scientific presentations and produced extensive extension and scientific publications (see "Products" for details. We continue to maintain our project website (SWDManagement.org) and conducted the first of four planned webinars on 25 January 2017, which was viewed live on 272 screens and the recording which has been viewed an additional 851 times as of this writing. What do you plan to do during the next reporting period to accomplish the goals?Objective 1: Implement and evaluate SWD management programs 1.1 Develop and implement grower-scale best management practices for SWD: We will add new best management strategies to on farm trials for 2018. 1.2 Build tools to measure SWD impact, predict losses, and suggest mitigation strategies:The next step is to use the NYS model as a base to develop interactive tools for other regions, staring with Maine and North Carolina. 1.3 Provide stakeholders with results, applications, and interpretation of project activities: We will continue to expand the project website, hold at least 3 more webinar sessions, and continue extension activities. Objective 2: Develop tactics and tools that predict SWD risk 2.1 Field validate and implement population models: Population models will be improved based on field trials and will ultimately be translated into interactive decision aids. 2.2 Determine sources of SWD populations between and during growing seasons: Genetic markers will be utlized to differentiate SWD populations within the US and track seasonal movement. 2.3 Develop monitoring tools that accurately estimate SWD populations and infestation risk: Based on 2017 results, new attractants will be screened in 2018. Objective 3: Optimize sustainable SWD management programs 3.1 Reduce reliance on insecticides in management programs:The Isaacs lab is conducting experiments in collaboration with other project partners to examine the effect of commonly used insecticides on different life stages of SWD. The generated data will be included in the population model to determine the effect of different insecticides on SWD populations. ? 3.2 Develop insecticide resistance detection, minimization, and management strategies: 3.3 Discover natural enemies capable of contributing to population reduction: USDA APHIS PPQ did not make permit request decisions until in July 2017, and the petitions were not approved, requiring more non-target tests with host fruits and some extra ecological studies on these parasitoids' temperature ranges. The requested studies are underway. In addition, one trip was made to collect more SWD parasitoids in South Korea in July 2017. A total of 15,000 Drosophila puparia were collected from wild Rubus and imported to the quarantine facilities at Berkeley. Over 400 individual wasps of the three major parasitoids emerged from this collection were added to maintain vigor of the quarantine colonies. 3.4 Reduce infestation rate in fruit post-harvest: In collaboration with the Isaacs lab, the Dong lab has been examining the repellent activities of various plant-derived volatiles against SWD adults. A total of eight volatiles exhibited strong repellency in the laboratory behavioral assays. Furthermore, the olfactory neurons that sense these volatiles have been identified using single sensillum recording. A manuscript based on these results is being prepared for publication. The Dong lab plan to work with Isaacs and Gut labs next summer to carry out field trials of these volatiles to assess the efficacy and to evaluate their potential in SWD management. 3.5 Develop genetic management tactics: We are currently making new driver lines that should express higher levels of tTA. In addition, three transgenic lines have been established with a female-specific promoter driving tTA expression.The strongest Cas9 lines will be used for future gene editing and for targeting gene constructs to specific locations in the genome.

IMPACT: 2016/09/01 TO 2017/08/31
What was accomplished under these goals? Obj 1: 1.1: On farm research and best management demonstration projects were conducted in each participating state during 2017. Best management practices under evaluation include using adult traps to time the start of insecticide application in locations where spring populations are low (northern US), and inclution of NuFilm P, and adjuvant, with registered insecticides. 1.2: We developed a bioeconomic model to evaluate the performance of alternative SWD management strategies at the farm level. We applied this model to a blueberry grower deciding how to best control a SWD infestation in New York State. Based on researchers, extension personnel, and growers' suggestions, we refined the interactive excel spreadsheet model regarding the costs of controlling SWD infestations. 1.3: See below. Obj 2: 2.1: Cooperators at Cornell University managed a multistate field experiment with project collaborators in North Carolina, Georgia, Maine, Michigan, and Oregon to assess mortality of winter morph and summer morph flies during 2016-2017. Cornell cooperators also conducted additional research in the winter-spring period of 2017 on the influence of alternative spring food hosts on adult survival and reproduction and habitat choices of winter morph flies and summer morph flies under field cage conditions. Cooperators at University of California, Davishave initiated studies of heat effects in a location with hot summer temperatures regime that appears to suppress population and in a coastal area with more mild temperature conditions and no summer population suppression utilizing a captive cohort demographic approach. 2.2: In partnership with cooperators at Cornell University, cooperators at the University of California, Davis have developed best practices for field fly collection that allows for extraction of high quality DNA which are being used by other project team members. UCD cooperators have generated version 2 of the SWD genome. The new SWD genome assembly and annotation is superior to version 1 (published in 2013) based on all quality control measures (number of scaffolds > 500bp, N50, number of spanned gaps between scaffolds) and will facilitate more accurate mapping of genetic variations from different geographical populations of SWD. We have now sequenced 165 SWD genomes at 15 to 20X coverage for a total of 33 geographical populations from 5 countries including the U.S. Within the U.S., our population sampling covers 9 states. 5 individuals were sequenced per sampling location. In the next 2 months (by November 2017), we plan to complete the genome sequencing portion. We have developed a custom bioinformatic pipeline to select SNP variants that can differentiate different SWD populations. Once genome sequencing for all geographical populations are completed this pipeline will identify SNP genotyping markers that will be used for the next stage of the project. 2.3: Cooperators at Rutgers are leading investigations of novel attractants for SWD. Leaf, fruit, yeast and fermentation volatiles, alone or in combination, are being tested versus the standard yeast/sugar mixture. This study is being conducted in regionally appropriate crop systems in six states. Cooperators at Michigan State University are leading a single-trap, multiple-release experiments which were first conducted in MIchigan during 2016 and are being repeated in 2017 in additional states. Michigan experiments during 2016 were conducted post-harvest in a large commercial cherry orchard. Results indicated that the maximum dispersal distance for SWD is 115m, plume reach for the trap is less than 5m and one trap is sampling 11.2 ac or 4.5 ha. Obj 3: 3.1: Cooperators at Michigan State University have completed field trials to test phagostimulants for enhancing the efficacy of insecticides in blueberry and some additional laboratory experiments are planned to assess the factors affecting the use of phagostimulants and have also initiated trials in collaboration with project partners in four other states to assess the rain fastness of insecticides alone and in combination with spray adjuvants. 3.2: Cooperators at Michigan State University and the University of Georgia have developed protocols for the monitoring of insecticides resistance in SWD populations using scintillation vials. A protocol was developed to monitor insecticides resistance using a discriminating dose (LC99x2) and is currently been validated nationally, a final protocol for use by extension agent and crop consultants will finalized by 2018. Cooperators at Michigan State University have isolated cDNA clones encoding the GABA receptor (rdl) and the nicotinic acetylcholine receptor (alpha 6 subunit), which are targets of fipronil and spinosad, respectively, in SWD adults. MSU has also established collaboration with Hunan Agricultural University in China, where insecticides are being used extensively for the control of SWD. Collaborators will monitor insecticide resistance and send genomic DNA of resistant individuals to for further sequence analysis. 3.3: Cooperators at the University of California, Berkeley coordinated national surveys of resident parasitions in six states through direct samples of fruits, fruit baiting traps and sentinel pupal traps. At least two larval parasitoids Leptopilina heterotoma and L. boulardi (Figitidae), and two pupal parasitoids Pachycrepoideus vindemiae (Pteromalidae) and Trichopria drosophilae (Diapriidae) emerged from field-collected fruits. The two larval parasitoids emerged from other drosophilids, not SWD, and laboratory tests further confirmed that they do not develop in SWD. The two pupal parasitoids emerged from various drosophilids including SWD but the parasitism was <10%. We evaluated three major larval parasitoids Asobara japonica (Braconidae), Leptopilina japonica and Ganaspis brasiliensis (Figitidae) collected during previous explorations in South Korea and China. These three larval parasitoids have been further evaluated for their biology and efficiency, including olfactory response towards SWD-infested fruits, efficiency in locating SWD in fruits or artificial diet, fecundity, egg maturation dynamic, host stage preference and suitability, functional response, preference and performance on different host species, as well as the outcomes of potential interspecific competition. All three parasitoids were also tested for their host specificity with a range of 24 non-target Drosophilidae species. A. japonica is more general whereas the host range of the other two larval parasitoid species is largely limited to species that are closely related to SWD. We selected G. brasiliensis and L. japonica for the approval of field release in North America and the petitions to release these two parasitoids were submitted in December 2016. 3.4: We have published results from initial cold storage experiments (Aly, et al. 2017) and will expand experiments in 2018. 3.5: Single component. Two strains FL19 and FL20, are 98 and 94% female lethal on diet without tetracycline. Females are fully viable on diet with tetracycline. Two component. 27 transgenic lines established carrying tetO-effector gene constructs. The effector genes were the proapoptotic genes hid, reaper and grim and also disco, a gene that regulates development and is lethal when widely expressed. When crossed with sry-tTA driver lines previously established, none of the effector genes were 100% lethal. Obj 3.5 Crispr/Cas9. Transgenic lines established that express Cas9 in the germline. When crossed with transgenic strains that express gRNA for the white gene, we observe a high frequency of germline mutations in the white gene (white eyes).

PUBLICATIONS: 2016/09/01 TO 2017/08/31
1. Type: Journal Articles Status: Published Year Published: 2017 Citation: Evans, R. K., M. D. Toews, and A. A. Sial. 2017. Diel periodicity of Drosophila suzukii (Diptera: Drosophilidae) under field conditions. PLoS ONE 12(2): e0171718. doi:10.1371/journal. pone.0171718
2. Type: Journal Articles Status: Published Year Published: 2017 Citation: Farnsworth, D., M. Bolda, R. Goodhue, J. Williams, and F. Zalom. 2017. Economic analysis of revenue losses and control costs associated with the spotted wing drosophila (Drosophila suzukii) in the California raspberry industry. Pest Manage. Sci. 73(6): 1083-1090.
3. Type: Journal Articles Status: Published Year Published: 2016 Citation: Abrieux A, J.C. Chiu. 2016. Oral delivery of dsRNA by microbes: beyond pest control. Communicative and Integrative Biology, 9(6): doi.org/10.1080/1942.
4. Type: Journal Articles Status: Published Year Published: 2017 Citation: Alnajjar, G., J. Collins, and F.A. Drummond. 2017. Behavioral and preventative management of Drosophila suzukii Matsumura (Diptera: Drosophilidae) in Maine wild blueberry (Vaccinium angustifolium Aiton) through attract and kill trapping and insect exclusion-netting. Intl. J. Entomol. Nematol. 3(1): 51-61.
5. Type: Journal Articles Status: Published Year Published: 2017 Citation: Diepenbrock, L.M., J.A. Hardin, H.J. Burrack. 2017. Season-long programs for control of Drosophila suzukii in southeastern United States blackberries. Crop Protection. 98: 149-156. http://doi.org/10.1016/j.cropro.2017.03.022
6. Type: Journal Articles Status: Published Year Published: 2017 Citation: Fraimout, A., Debat, V., Fellous, S., Hufbaurer, R.A., Foucaud, J., Pudlo, P., Marin, J., Price, D.K., Cattel, J., Chen, X., Drpra, M., Byyck, P., Guedot, C., Kenis, M., Kimura, M.T., Loeb, G., Loiseau,A., Martinez-Sanudo, I., Pascual, M., Richond, M., hearer, P., Singh, N., Tamura, K., Xuyereb, A., Zhang, J., and Estoup, A. 2017. Deciphering the routes of invasion of Drosophila suzuki by means of abc random forest. Molecular Biology Evolution, doi:10.1093/molbev/msx050.
7. Type: Journal Articles Status: Published Year Published: 2017 Citation: Ka?ar G, Wang XG, Biondi A, Daane KM 2017. Linear functional response by two pupal Drosophila parasitoids foraging within single or multiple patch environments. PLoS ONE https://doi.org/10.1371/journal.pone.0183525
8. Type: Journal Articles Status: Published Year Published: 2017 Citation: Mishra, R., J. C. Chiu, G. Hua, N. R. Tawari, M. J. Adang, and A. A. Sial. 2017. High throughput sequencing reveals Drosophila suzukii responses to insecticides. Insect Science 1-19, DOI 10.1111/1744-7917.12498.
9. Type: Journal Articles Status: Published Year Published: 2017 Citation: Rosensteel, D. O. and A. A. Sial. 2017. Efficacy of insecticides against Drosophila suzukii in rabbiteye blueberries. Arthropod Management Tests, 2017. 42 (C13).
10. Type: Journal Articles Status: Published Year Published: 2017 Citation: Rosensteel, D. O. and A. A. Sial. 2017. Efficacy of insecticides against Drosophila suzukii in Southern highbush blueberries. Arthropod Management Tests, 2017. 42 (C13).
11. Type: Journal Articles Status: Published Year Published: 2017 Citation: Swoboda-Bhattarai, K.S., D.R. McPhie, and H.J. Burrack. 2017. Reproductive Status of Drosophila suzukii (Diptera: Drosophilidae) Females Influences Attraction to Fermentation-Based Baits and Ripe Fruits. Journal of Economic Entomology. 10.1093/jee/tox150.
12. Type: Journal Articles Status: Published Year Published: 2016 Citation: Wallingford, A., Lee, J, Loeb, G. 2016. The influence of temperature and photoperiod on the reproductive diapause and cold tolerance of spotted-wing drosophila, Drosophila suzukii (Matsumura). Entomologia Exerimentalis et Applicata, 159: 327-337. DOI: 10.1093/jee/tow116.
13. Type: Journal Articles Status: Published Year Published: 2016 Citation: Wang XG, Ka?ar G, Biondi A, Daane KM (2016) Life-history and host preference of the pupal parasitoid Trichopria drosophilae of spotted wing drosophila. BioControl 61:387-397.
14. Type: Journal Articles Status: Published Year Published: 2017 Citation: Woltz JM, Wiman NG, Lee JC. 2017. Two Pests Overlap: Drosophila suzukii (Diptera: Drosophilidae) Use of Fruit Exposed to Halyomorpha halys (Hemiptera: Pentatomidae). Journal of Economic Entomology. DOI: 10.1093/jee/tox156
15. Type: Other Status: Published Year Published: 2017 Citation: Leach, H. and Isaacs, R. 2017.Cultural controls to minimize SWD infestation. Newsletter of the North American Raspberry and Blackberry Association. July 2017.
16. Type: Other Status: Published Year Published: 2017 Citation: Wilson, J. L. Gut, N. Rothwell, M. Haas, E. Pochubay, K. Powers, M. Whalon and J. Wise. 2017. Managing Spotted Wing Drosophila in Michigan Cherry. http://www.ipm.msu.edu/uploads/files/SWD/SWDManagementGuideCherries.pdf
17. Type: Other Status: Published Year Published: 2017 Citation: Wilson, J. R, Isaacs and L. Gut. 2017. Michigan spotted wing Drosophila reports 8 June through 1 Aug, 2017. MSU Extension News for Agric. Total of 19 weekly reports.
18. Type: Other Status: Published Year Published: 2017 Citation: Wallingford, A, Elsensohn, J., Swododa-Bhattarai, Burrack, H., and Loeb, G. 2017. Local populations of spotted wing drosophila in wild host plants. NYS Berry Growers Association newsletter, Issue 1, April 2017, Pages 1-3.
19. Type: Other Status: Published Year Published: 2016 Citation: Wallingford, A. and Loeb, G. 2016. Spotted wing drosophila winter biology. NY Fruit Quarterly. NY Fruit Quarterly 24 (3): 11-13.
20. Type: Other Status: Published Year Published: 2016 Citation: Wang XG, Jones J, Nance A, Hutchins J, Miller B, Miller VM, Hoelmer KA, Buffington M, Daane KM (2017) Investigating biological controls to suppress spotted wing drosophila populations. 2016 Annual Crop Report, California Cherry Board, January 5 2017. pp. 1-10.
21. Type: Other Status: Published Year Published: 2017 Citation: Yarborough, D., F.A. Drummond, and J.A. Collins. 2017. Insect control guide for wild blueberries. Univ. Maine Coop. Ext. Fact Sheet No. 209, UMaine Extension No. 2001, 14 pp.
22. Type: Other Status: Published Year Published: 2017 Citation: Cote, J., D. E. Yarborough, F.A. Drummond, and J.A. Collins. 2017. Maine Wild blueberry pesticide chart ? 1 of 3. http://umaine.edu/blueberries/files/2010/05/2013-ME-Wild-BB-Pesticide-Chart-Ins ecticides.pdf
23. Type: Other Status: Published Year Published: 2017 Citation: Drummond, F.A., J. Collins, and E. Ballman. 2017. Spotted Wing Drosophila: Pest Biology and IPM Recommendations for Wild Blueberries. Univ. Maine Coop. Ext. Fact Sheet 210, 6 pp. https://extension.umaine.edu/blueberries/factsheets/insects/210-spotted-wing-dr osophila/
24. Type: Other Status: Other Year Published: 2017 Citation: Michel, C., C. Rodriguez-Saona, A.L. Nielsen, and D. Polk. 2017. La mosca de alas manchadas: Una plaga de frutos peque?os en Nueva Jersey. Fact Sheet 1266. Rutgers Cooperative Extension.
25. Type: Other Status: Published Year Published: 2016 Citation: Sial, A. A. 2016. Spotted Wing Drosophila (SWD) Identification, Monitoring, and Management Handouts. UGA Blueberry blog, 27 April 2016. http://blog.caes.uga.edu/blueberry/2016/04/swd-handouts/
26. Type: Other Status: Published Year Published: 2016 Citation: Sial, A. A. 2016. Insecticide Regime for SWD Control on Blueberries. UGA Blueberry blog, 27 April 2016. http://blog.caes.uga.edu/blueberry/2016/04/insecticide-regime-for-swd/
27. Type: Other Status: Published Year Published: 2016 Citation: Sial, A. A. 2016. Spotted Wing Drosophila Identification, Monitoring, and Management in Georgia Blueberries. UGA Blueberry blog, 27 April 2016. http://blog.caes.uga.edu/blueberry/2016/04/spotted-wing-drosophila/
28. Type: Journal Articles Status: Published Year Published: 2016 Citation: Brown, Z.S. (2017). The Economics, Regulation and International Implications of Gene Drives in Agriculture. Choices, Quarter 2.
29. Type: Other Status: Published Year Published: 2016 Citation: Wiman, NG, J Pscheidt, and E Peachey. 2016-17. Cherry pest management guide for the Willamette Valley. Oregon State University Extension Service Publication EM 8329.
30. Type: Websites Status: Published Year Published: 2017 Citation: Palmer, G.F. and H.J. Burrack. 2017. Blueberry insect monitoring reports. 14 April through 23 June 2017. Total of 11 reports posted at blueberries.ces.ncsu.edu. https://blueberries.ces.ncsu.edu/tags/blueberry-scouting-reports-2017/
31. Type: Other Status: Published Year Published: 2017 Citation: Southern Regional Blueberry Integrated Management Guide. 2017. Editor: H.J. Burrack. Section editor: P. Brannen, B. Cline, F. Hale, A. Sial, M. Czarnota, K. Jennings, D. Lockwood. Editor beginning 2011.
32. Type: Other Status: Published Year Published: 2017 Citation: Southeast Region Caneberry Integrated Management Guide. 2017. Editor, P. Brannen. Section editors: G. Schnabel, D. Horton, H.J. Burrack, D. Pfeiffer, K. Jennings, W. Mitchem, D. Lockwood, G. Fernandez.
33. Type: Other Status: Published Year Published: 2017 Citation: Southeast Regional Strawberry Integrated Management Guide. 2017. Editor: F.J. Louws and Carol Hicks. Section editors: C. Johnson, G. Schnabel, K. Fontenot, H.J. Burrack, K. Jennings, and W. Mitchem.
34. Type: Other Status: Published Year Published: 2017 Citation: Burrack, H.J. 2017. Strawberry insect management. North Carolina Cooperative Extension Service. AG-1. (http://content.ces.ncsu.edu/north-carolina-agricultural-chemicals-manual)
35. Type: Other Status: Published Year Published: 2017 Citation: Burrack, H.J. and W.O. Cline. Blueberry management program. 2017. North Carolina Cooperative Extension Service. AG-1. (http://content.ces.ncsu.edu/north-carolina-agricultural-chemicals-manual)
36. Type: Other Status: Published Year Published: 2017 Citation: Burrack, H.J. and W.O. Cline. 2017. Caneberry management program. . North Carolina Cooperative Extension Service. AG-1. (http://content.ces.ncsu.edu/north-carolina-agricultural-chemicals-manual)
37. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Abrieux,A. *, J.C. Chiu, Investigating population structure and dispersal of Spotted Wing Drosophila in the United States, Pacific Branch ESA Meeting, Portland, OR, USA (April 15, 2017)
38. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Andrews*, H, VM Walton, S Mermer and NG Wiman. Sweetening the dead: phagostimulants and insecticide efficacy for SWD. Orchard Pest and Disease Management Conference. Portland, OR. 11 Jan. 2017
39. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Bezerra Da Silva*, C.S., Park, K.R., Walton, V.M. 2017 (1h) ? Sustainable management of SWD. Biocontrol USA West 2017 Conference & Expo, Reno, NV
40. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Bezerra Da Silva*, C.S., Park, K.R., Walton, V.M. 2017 (1h) ? Larval competition and pupation behavior of the SWD. 101st Annual Meeting of the PBESA, Portland, OR.
41. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Burrack, H.J., L.M. Diepenbrock, K. Swoboda-Bhattarai, Y. Zheng, and A. Sial. 29 September 2016. A season-long strategy to manage spotted wing drosophila. International Congress of Entomology. Orlando, FL.
42. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Chiu, J.C.*, Leveraging genomic approaches to study seasonal biology of Drosophila suzukii, 10th Arthropod Genomics Symposium, University of Notre Dame, South Bend, IN. (June 9, 2017)
43. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Chiu, J.C.*, Accelerating research on Spotted Wing Drosophila management using genomic technologies, Plant and Animal Genome XXV Conference, San Diego, CA, USA (January 17, 2017)
44. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Chiu, J.C.*, Delivery of dsRNA biopesticide using engineered microbes, Plant and Animal Genome XXV Conference, San Diego, CA, USA (January 14, 2017)
45. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Chiu, J.C.*, Implications of circadian and seasonal biology for sustainable agriculture: Spotted Wing Drosophila as a case study, Department of Entomology, North Carolina State University, Raleigh, NC (November 14, 2017)
46. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Chiu, J.C.*, Implications of circadian and seasonal biology for sustainable agriculture: Spotted Wing Drosophila as a case study, Department of Entomology, Pennsylvania State University, State College, PA (November 4, 2017)
47. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Chiu, J.C.*, Peter Shearer, Vaughn Walton, Genome-enabled Spotted Wing Drosophila Research, International Congress of Entomology, Orlando, FL (October 29, 2016)
48. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Daane KM*, Wang XG, Hoelmer KA, Miller B, Guerrieri E, Giorgini M. Foreign exploration for Drosophila suzukii parasitoids in China and South Korea, depending on which one would fit better into your symposium. The 101st ESA Pacific Branch meeting, Portland, Oregon, 2-5 April 2017.
49. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Elsensohn, J., Z.S. Brown, and H.J. Burrack. 27 September 2016. New kids on the block: Regulatory issues around emerging pests and emerging technologies. International Congress of Entomology. Orlando, FL.
50. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Burrack, H.J. 6 July 2017. Management of Drosophila suzukii in the United States. IX Seminario Brasileiro sobre Pequenas Frutas (9th Brazilian Seminar on Small Fruits). Presented remotely. Vacaria, RS, Brazil.
51. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Burrack, H.J., J. Chiu, K. Daane, L. Diepenbrock, R. Isaacs, G. Loeb, C. Rodriguez-Saona, A. Sial, K. Swoboda-Bhattarai, and V. Walton. 25 May 2017. Drosophila suzukii invasions and options for management. Third FAO/IAEA International Conference on Area-Wide Management of Insect Pests: Integrating the sterile insect and related nuclear and other techniques. Vienna, Austria.
52. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Gomez, M.I. 2017 Empire State Producers Expo "SWD Management and Impact on Profits", Syracuse, NY, January 17, 2017.
53. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Hoelmer, K. 2016. "The biological control of Spotted Wing Drosophila suzukii, a potential pest for Chile", presented by K. Hoelmer, 3rd Chilean Symposium on Biological Control, Chillan, Chile.
54. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Kirkpatrick, D. *, L. Gut and J. Miller: Improving monitoring tools for spotted wing drosophila, Drosophila suzukii. Sep 2016. XXV International Congress of Entomology, Orlando, Florida.
55. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Kirkpatrick,D. * and L. Gut: Improving monitoring tools for spotted wing drosophila, Drosophila suzukii. Sep 2016. IOBC-WPRS 9th International Conference on Integrated Fruit Production, Thessaloniki, Greece.
56. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: *Loeb, G, Cowles, R. 2016. Drosophila suzukii alternate host and spatial distribution and improvement of pesticide efficacy. Invited speaker as part of symposium entitled ?International perspectives contribute toward a clearer understanding of Drosophila suzukii? organized by V. Walton, G. Anfora, N. Wiman, and Ash Sia at the 2016 International Congress of Entomology in Orlando, Fl.
57. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Sial, A. A. A tiny fly with a global impact: Developing sustainable IPM programs. Seminar at Cairo University, Egypt, 27 December 2016. (International)
58. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Walton, V.M., N.G. Wiman, D.T. Dalton, B. Miller, R.M. York, P.W. Shearer, S.L. Tochen, L.K. Tanigoshi, B.S. Gerdeman, J.C. Lee, C. Ioriatti, G. Anfora, A. Grassi, R. Isaacs, and H.J. Burrack. 29 September 2016. Biology, management, and population modeling of Drosophila suzukii in western North American production regions. International Congress of Entomology. Orlando, FL.
59. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Wang XG*, Daane KM, Biondi A, Jones J, Nance A. Evaluations of native Asian parasitoids for biological control of Drosophila suzukii. The 101st ESA Pacific Branch meeting, Portland, Oregon, 2-5 April 2017.
60. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Wang XG, Daane KM, Mille B, Walton VW, Serrato M*. Comparison of the thermal performance between two resident pupal parasitoids of Drosophila suzukii. The 101st ESA Pacific Branch meeting, Portland, Oregon, 2-5 April 2017.
61. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Brown, Z.S. (2017). Voluntary programs to encourage compliance with refuge regulations for pesticide resistance management: evidence from a quasi-experiment. Presented at 2016 AAEA Annual Meeting.
62. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Roh, H. and Z.S. Brown (2017). Adaptive resistance management with uncertain fitness costs. Presented at 2016 AERE Annual Meeting.
63. Type: Conference Papers and Presentations Status: Other Year Published: 2017 Citation: Diepenbrock, L., A. Abrieux, H. Burrack, J. Chiu, K. Daane, L. Gut, R. Isaacs, G. Loeb, C. Rodriguez-Soana, A. Sial, K. Swoboda-Bhattarai, V. Walton. 18 September 2017. Developing integrated pest management programs for spotted wing drosophila (Drosophila suzukii). 48th Annual Australian Entomological Society Conference. Terrigal, NSW, Australia.
64. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Rodriguez-Saona, C. 2016. Impacto de una plaga invasiva en el Manejo Integrado de Plagas: Drosophila de alas manchadas en ar?ndano en los EE.UU (Impact of an invasive species on Integrated Pest Management: Spotted wing drosophila in blueberries in the USA). Servicio Nacional de Sanidad Agraria (SENASA). Direcci?n de Sanidad Vegetal. La Molina, Lima, Per?.

PROGRESS: 2015/09/01 TO 2016/08/31
Target Audience:Target audience:Growers of crops impacted by spotted wing drosophila (SWD) and the extension personnel that serve them. Our primary efforts are directed at US cherry, blueberry, blackberry, raspberry, and strawberry growers, but we also engagewith growers of other crops that have SWD concerns. Our project team has also developed international connections ito facilitate information transfer to other parts of the world where SWD is a significant pest. Efforts We participated in a total of 37 extension events during the first year of the project, directly reached at least 1200 attendees. Burrack, HJ. SRSFC Regional Agent Training. 14 Sept2016. Key insect pests and their management in berry crops. Shelby, NC. 23 attendees. Drummond, F. Summer Wild Blueberry Field Day. 20 Jul2016. Monitoring spotted wing drosophila in relation to action thresholds. Jonesboro, ME. Walton, V. Blueberry Field Day. 6 Jul2016. NWREC, Aurora, OR. 100 attendees. Drummond, F. Twilight Wild Blueberry Growers Meeting. 30 Jun2016. Management of spotted wing drosophila in wild blueberry. Orland, ME. Drummond, F. Twilight Wild Blueberry Growers Meeting. 29 Jun2016. Management of spotted wing drosophila in wild blueberry. Jonesboro, ME. Drummond, F. Twilight Wild Blueberry Growers Meeting. 28 Jun2016. Management of spotted wing drosophila in wild blueberry. Warren, ME. Walton, V. Wasco Co. Pre-Harvest day for cherry. 31 May 2016. The Dalles, OR. 80 attendees. Isaacs, R. SWD management workshop for berry growers. May 2016. Port Sheldon, Michigan. 20 growers. Walton, V. Oregon Wine Research Instfield scouting workshop. 4 May 2016. Milton-Freewater, OR. 50 attendees. Walton, V. Wasco County cherry breakfast growers meeting. 29 Apr2016. Spotted Wing Drosophila management. The Dalles, OR. 60 attendees. Burrack, HJ. North American Raspberry and Blackberry Association. 3 Mar2016. Reducing reliance on insecticides to manage spotted wing drosophila. Williamsburg, VA. 50 attendees. Burrack, HJ. North American Raspberry and Blackberry Association. 3 Mar 2016. Fundamentals of insect management in blackberries. Williamsburg, VA. 45 attendees. Burrack, HJ. NC Commercial Blackberry & Raspberry Growers Association. 19 Feb2016. A guide to season-long insect control. Shelby, NC. 34 attendees. Loeb, G. Hudson Valley Commercial Fruit Growers' School. 18 Feby 2016. Research update: Invasive insects in berry crops. Hudson, NY. 30 attendees. Burrack, HJ. Bladen County Blueberry Meeting. 16 Feb2016. Putting it all together: developing an SWD management strategy focused on reducing pesticides. Elizabethtown, NC. 150 attendees. Walton, V. Blue Mountain Horticultural Society. Feb10, 2016. Managing Red Blotch, Spotted Wing Drosophila and Brown Marmorated Stink bug. Portland OR. 35 attendees. Burrack, HJ. Mid Atlantic Fruit & Vegetable Conference. 4 Feb2016. A national research effort to manage spotted wing drosophila and recent advances in biology and management. Hershey, PA. 95 attendees. Loeb, G. Mid Atlantic Fruit & Vegetable Conference. 3 Feb2016. Spotted wing drosophila: When do I really need to start spraying? Hershey, PA. 100 attendees. Daane KM. California Cherry Research Review. 26 Jan2016. Investigating biological controls to suppress spotted wing drosophila populations. Stockton, CA. Walton, VM. Oregon Blueberry Conference, Portland Oregon, 25 Jan2016. Improved Spray Technologies for SWD. 200 attendees. Lovett, WE, AA Sial, JL Jacobs, BK Gautam, and BA Little. 2016 UGA Cooperative Extension Winter Conference, 12-14 Jan 2016. Impact of simulated rainfall on the effectiveness of insecticides against spotted wing drosophila in blueberries. Rock Eagle, GA. Sial, AA. 2016 UGA Cooperative Extension Winter Conference, 12-14 Jan 2016. Spotted wing drosophila (SWD): A threat to small and stone fruit production. Rock Eagle, GA. Evans, RK and Sial, AA. Southeastern Regional Fruit and Vegetable Conference, 7-10 Jan 2016. Effect of abiotic factors on biology, behavior, and reproduction in Drosophila suzukii (Diptera: Drosophilidae). Savannah, GA. Gautam, BK and Sial, AA. Southeastern Regional Fruit and Vegetable Conference, 7-10 Jan 2016. Effect of temperature on acute toxicity of insecticides to spotted wing drosophila, Drosophila suzukii. Savannah, GA. Grant, JA and Sial, AA. Southeastern Regional Fruit and Vegetable Conference, 7-10 Jan 2016. Investigating native flora as a potential source of spotted wing drosophila infestations in Georgia blueberries. Savannah, GA. Little, BA and Sial, AA. Southeastern Regional Fruit and Vegetable Conference, 7-10 Jan 2016. Effect of spray solution pH on efficacy and residual activity of insecticides against Drosophila suzukii (Diptera: Drosophilidae). Savannah, GA. Sial, AA. Southeastern Regional Fruit and Vegetable Conference, 7-10 Jan 2016. Blueberry Entomology Update. Savannah, GA. Burrack, HJ. Southeastern Fruit & Vegetable Conference. 7 Jan2016. Year-round management strategies for insect pests in blackberries. Savannah, GA. 80 attendees. Burrack, HJ. North American Strawberry Growers Association. 7 Jan2016. Strawberry pest management post spotted wing drosophila. Savannah, GA. 85 attendees. Evans, R. K. and Sial, A. A. GA Blueberry Growers Meeting - Annual Blueberry Update, 6 Jan 2016. Effect of abiotic factors on biology, behavior, and reproduction in Drosophila suzukii (Diptera: Drosophilidae). Alma, GA. Gautam, B. K. and Sial, A. A. GABlueberry Growers Meeting - Annual Blueberry Update, 6 Jan 2016. Effect of temperature on acute toxicity of insecticides to spotted wing drosophila. Alma, GA. Grant, J. A. and Sial, A. A. GABlueberry Growers Meeting - Annual Blueberry Update, 6 Jan 2016. Investigating native flora as a potential source of spotted wing drosophila infestations in Georgia blueberries. Alma, GA. Little, B. A. and Sial, A. A. GABlueberry Growers Meeting - Annual Blueberry Update, 6 Jan 2016. Effect of spray solution pH on efficacy and residual activity of insecticides against Drosophila suzukii (Diptera: Drosophilidae). Alma, GA. Sial, AA. GABlueberry Growers Meeting - Annual Blueberry Update, 6 Jan 2016. Biology and management of spotted wing drosophila: 2015 Research Update. Alma, GA. Gut, L. Michigan State Horticultural Society. Dec 2015. Results from spotted wing drosophila monitoring network and lure study. Grand Rapids, MI. Gut, L. SWD Summit. Effectiveness of SWD traps and baits. Nov 2015. Northwest Michigan Horticultural Research Center. Walton, V. 5 October 2015. Managing invasive insects in tree crops and small fruit. Roseburg, OR. 35 attendees. International extension activities We coordinated a series of webinars presented to a team from the Plant Health Division of Peruvian Ministry of Agriculture (approximately 12 attendees each session) who were in the process of formulating detection protocols and regulations for spotted wing drosophila (SWD, Drosphila suzukii) from 20-22 April 2016. As of the present, SWD has yet to be detected in Peru. Three sessions were presented covering SWD population modeling (V. Walton), monitoring tools (C. Rodriguez-Saona), and host preference and risk (H. Burrack and R. Isaacs). Sessions were simultaneously translated from English to Spanish and recorded for future use with bilingual stakeholders. Project director Burrack volunteer with theUSDAID Partners for the Americas Farmer-to-Farmer Program inRio Grande do Sul, Brazil from 29 November-15 December 2015.The purpose of this visit was to share knowledge about SWDbiology and management with scientists and farmers in southern Brazil, where ithas been present since 2013. Burrackvisited 14 farms producing strawberries, blackberries, raspberries, blueberries, and grapes, which was facilitated by Dr. Regis Sivori, EMBRAPA Uva e Vinho (Grape and Wine), Vacaria, RS. Dr. Sivori and Burrack presented two workshops, at EMBRAPA Bento Goncalves and Vacaria attended by 74 area extension (Ematar) personnel, scientists, and others. Changes/Problems:Co PI Peter Shearer, formerly of Oregon State University, is no longer a participant in this project. We have made an effort to include additional representation from the cherry industry in our stakeholder advisory board and added Dr. Nik Wiman as a co PI to the project. Dr. Wiman will cover activities in Oregon proposed by Dr. Shearer. What opportunities for training and professional development has the project provided?See "Target Audience" for detailed information on training and professional development. How have the results been disseminated to communities of interest?See "Target Audience", "Products", and "Other Products" for detailed information on information dissemination. What do you plan to do during the next reporting period to accomplish the goals?Objective 1.1: Develop improved BMPs during our annual project meeting (Feb 2017), and employ these at on farm locations during the growing season. Objective 1.2: Modify and apply the model to other crops and states; and extend the model to consider regional and national SWD diffusion and optimal actions to control infestations. Objective 1.3:Develop excel spreadsheet for bioeconomic models inother crops and states/regions; and start work on the online calculator tool for optimal SWD control. Objective 2.1: Population model validation will continue, and once validated, models will be integrated into project website. Objective 2.2: SNPs will be indenified at the different geographic scales targeted (state, region, local). Objective 2.3: New attractants will be idenified in collaboration with the project team during winter 2017 and will be tested in field trials. Range of attraction trials will be repeated in NC, GA, NJ, and OR. Objective 3.1: Standard trials for field insecticide experiments will be developed, implemented, and shared via the project website. Field experiments will continue and include new materials and application methodologies. Objective 3.2: Bioassay methods will be refined, and resistance screening will be conducted in additional locations. Objective 3.4:Future efforts will focus on how red spectrum reflectance differs between infested and uninfested blueberries in order to improve sorting technologies. Objective 3.5: Developed strains will be screened against laboratory and wild type lines for mating competitiveness and other fitness measures. A risk assessment exercise intended to identify potential end points (benefits and hazards) associated with genetically modified SWD will be conducted at the Feb 2017 annual project meeting. This risk assesment is intended to gather information on end points, rank them in relative importance based on stakeholder feedback, and compare this prioritized list to the criteria used in regulatory processes and research prioritization.

IMPACT: 2015/09/01 TO 2016/08/31
What was accomplished under these goals? Objective 1.1: Atotal of 29on farm research locations were established across 7 states. Sites were located on cherry, blueberry, blackberry, and raspberry farms. Two best management practices (BMPs) were assessed during the 2016 growing season. Locations growing blueberries and other low risk crops assessed whether currently available monitoring traps could be used to delay the start of insecticide applications as compared to starting weekly applications once fruit begin to ripen, as is currently recommended. Locations growing higher risk crops, including blackberries, raspberries, and late season blueberries, assessed whether spray programs including reduced risk insecticides were as effective at preventing infestation as those containing only organophosphate and pyrethroid insecticides. Pesticide residue samples and secondary pest assessment were conducted in addition to SWD infestation measurements. In general, there were no differences in BMP vs grower standard fields, suggesting that these practices can be recommended to growers with minimal risk of increased infestation. Objective 1.2: During 2016, we developed a farm level bioeconomic model to evaluate the cost-effectiveness of alternative SWD control strategies for a blueberry farm; we built a baseline Excel spreadsheet model for blueberries in the Northeast to estimate economic impacts of SWD infestation under alternative scenarios; we presented results at the annual conference of the Agricultural and Applied Economics Association. Objective 1.3:A project website (SWDManagement.org) has been developed to house information specific to our project activities. We conducted a series of experiments that determined that commercial scale optical blueberry sorters can remove over 40% of second instar infested fruit, but not significant amounts of first instar or egg infested fruit.We next conducted a series of experiments that determined that commercial scale optical blueberry sorters can remove over 40% of second instar infested fruit, but not significant amounts of first instar or egg infested fruit. Objective 2.1: Fly trap capture data have been collected at all on farm locations (Obj 1.1) to be used in validation of an existing population model. Results from Obj 2.2 are being incorporated to refine model predictions. Objective 2.2: SWD samples from all participating states have been collected and are being sequenced by the Chiu laboratory in order to develop SNPs useful in detecting sources of populations between states. In addition, detailed samples have been collected OR for use in developing markers to distinguish populations between regions within a state and from NC for use in developing markers to distinguish populations between different time points during the year. Experiment to assess the effects of high summer and low winter temperatures on fly survival have been conducted and will be repeated in 2017. Objective 2.3: All the chemosensory genes (olfactory and gustatory) were annotated in the D. suzukii and we have identified a set of gene lineages that have expanded uniquely, and some that were also unique losses. We are in the process of functional characterization.?Field trials comparing four newly developed volatile attractants, including those identified by our work, to current standards were conducted at 11 locations throughout the US. The results of these trials suggested that yeast and sugar solutions remain more attractive than other odors, although volatiles from fruit appeared more selective for female SWD. A single-trap, multiple-release experimental design is being used to measure the plume reach and maximum dispersal distance for SWD. These measures can be used to calculate the trapping area. The first releases were conducted in a 2-acre tart cherry orchard. The proportion of released flies recaptured was about 2%. It appears that SWD flies move a substantial distance, over 200 meters. It is unclear at this point if flies move randomly or are aided by the wind. A new set of releases are being conducted in a large cherry orchard, allowing us to release flies several hundred meters away from a central trap. Objective 3.1: We have initiated field trials to test phagostimulants for enhancing the efficacy of insecticides in blueberry and raspberry. We have also conducted field trials comparing different application methods (sprayers) in GA. A key future goal is the development of standard methods for field trials that can be employed beyond our project. Objective 3.2:Protocols for resistance assays have been developed in collaboration with UGA and are being tested this fall to compare the vial method with previously-used Petri dish methods. In support of the studies of insecticide resistance, the Dong lab have isolated partial cDNA clones, but no full-length cDNA clones. Further work is planed to clone full-length cDNA and then express sodium channels in Xenopus oocytes for functional and pharmacological analyses. Objective 3.3: We have dermined the composition of indigenous parasitoids that can attack SWD and their effectivenessin CA, OR, NY, GA, and NC,where we have found only two generalist pupal parasitoids that can readily attack SWD. One larvalparasitoid was collected in NC. Both pupal parasitoids have been evaluated in details for their biology on SWD as well as their relative effectiveness and potential interactions in laboratory (see "Products" for publication), and we plan to evaluate their effectiveness under field conditions. Due to the lack of larval indigenous parasitoids, our major aim of this project is to discover and introduce specialized larval parasitoids from SWD's native range in Asia. Currently, three larval parasitoids previously introduced from South Korea have been systematically evaluated in the quarantine for their effectiveness against SWD and host specificity. Two of them have been identified as effective and more host-specific species. A petition to release both larval parasitoids have been submitted. In July 2016, five trips (two by Kent Daane and Kim Hoelmer to South Korea, three by Kent Daane, Kim Hoelmer, and Xingeng Wang to China) weremade to collect SWD parasitoids in South Korea and China, and totally about 12,000 and 13,000 SWD have been imported from South Korea and China, respectively. Currently, we are screening parasitoids emerged from collected SWD in the quarantine. New parasitoids will be identified and evaluated for their effectiveness and host specificity. Objective 3.4:We published results of laboratory experiments determining that storage at temperatures lower than 35F reduced egg and third instar SWD survival in blueberries and raspberries and essentially stopped development of all immature life stages (Aly et al. 2016).We also conducted a series of experiments that determined that commercial scale optical blueberry sorters can remove over 40% of second instar infested fruit, but not significant amounts of first instar or egg infested fruit. Objective 3.5:Male-only strains. Transgenic lines have been made with sry-tTA and Sxl-tTA "driver" constructs. Transgenic lines have been made with tetO-reaper, tetO-grim, tetO-hid and tetO-disco effectors. The lines are being bred to homozygosity. We were unable to make transgenic lines with the tetO-hid[ala5] mutant, which appears to have too high lethality in D. suzukii. CRISPR/Cas9. Transgenic lines have been made with vasa-Cas9 and nanos-Cas9 that should express Cas9 in the female germline. we also have lines with betatub-Cas9 that should express Cas9 in the male germline. Transgenic lines have been made with U6-w gRNA and U6-Sxl gRNA that should express guide RNAs for the white and Sex lethal genes. Lines are being bred to homozygosity.

PUBLICATIONS: 2015/09/01 TO 2016/08/31
1. Type: Journal Articles Status: Accepted Year Published: 2016 Citation: Aly, MFK, DA Kraus, and HJ Burrack. Accepted. Effects of post-harvest cold storage on the development and survival of immature Drosophila suzukii (Matsumura) in artificial diet and fruit. Journal of Economic Entomology.
2. Type: Journal Articles Status: Published Year Published: 2016 Citation: Asplen MK, G Anfora, A Biondi, DS Choi, D Chu, KM Daane, P Gibert, AP Gutierrez, KA Hoelmer, WD Hutchison, R Isaacs, ZL Jiang, Z K?rp?ti, M T Kimura, M Pascual, CR Philips, C Plantamp, L Ponti, G V?tek, H Vogt, VM Walton, YY, L Zappal?, and N Desneux 2015. Invasion biology of spotted wing Drosophila (Drosophila suzukii): a global perspective and future priorities. Journal of Pest Science. 88: 469-494.
3. Type: Journal Articles Status: Published Year Published: 2016 Citation: Daane KM, X Wang, A Biondi, B Miller, JC Miller, H Riedl, PW Shearer, E Guerrieri, M Giorgini, M Buffington, K van Achterberg, Y Song, T Kang, H Yi, C Jung, DW Lee, BK Chung, KA Hoelmer, and VM Walton. 2016. First exploration of parasitoids of Drosophila suzukii in South Korea as potential classical biological agents. Journal of Pest Science. 89(3): 823-835. DOI 10.1007/s10340-016-0740-0
4. Type: Journal Articles Status: Published Year Published: 2016 Citation: Diepenbrock, LM, KA Swoboda-Bhattarai, and HJ Burrack. 2016. Oviposition preference, fidelity, and fitness of Drosophila suzukii in a co-occurring crop and non-crop host system. Journal of Pest Science. 10.1007/s10340-016-0764-5
5. Type: Journal Articles Status: Published Year Published: 2016 Citation: Diepenbrock, LM and HJ Burrack. 2016. Variation of within-microhabitat use by Drosophila suzukii (Diptera: Drosophilidae) in blackberry. Journal of Applied Entomology. DOI: 10.1111/jen.12335
6. Type: Journal Articles Status: Published Year Published: 2016 Citation: Hamby KA, DE Bellamy, JC Chiu, JC Lee, VM Walton, NG Wiman, and A Biondi. 2016. Biotic and abiotic factors impacting development, behavior, phenology, and reproductive biology of Drosophila suzukii. Journal of Pest Science. 1-15.
7. Type: Journal Articles Status: Published Year Published: 2016 Citation: Haye T, P Girod, AGS Cuthberstson, XG Wang, KM Daane, K Hoelmer, C Baroffio, J Zhang, and N Desneux. 2016. Current SWD IPM tactics and their practical implementation in fruit crops across different regions around the world (a review). Journal of Pest Science 89(3): 643-651.
8. Type: Journal Articles Status: Published Year Published: 2016 Citation: Hickner P, C Rivaldi, C Johnson, M Siddappaji, G Raster, and Z Syed. 2016. The making of a pest: Insights from the evolution of chemosensory receptor families in a pestiferous and invasive fly, Drosophila suzukii. BMC Genomics. Aug 17;17:648. doi: 10.1186/s12864-016-2983-9.
9. Type: Journal Articles Status: Submitted Year Published: 2016 Citation: Evans RK, MD Toews, and AA Sial. 2016. Diel periodicity of Drosophila suzukii (Diptera: Drosophilidae) under field conditions. PLOS ONE (Submitted)
10. Type: Journal Articles Status: Published Year Published: 2016 Citation: Lee JC, DT Dalton, KA Swoboda-Bhattarai, DJ Bruck, HJ Burrack, BC Strik, JM Woltz, and VM Walton. 2015 Characterization and manipulation of fruit susceptibility to Drosophila suzukii. Journal of Pest Science. 10.1007/s10340-015-0692-9.
11. Type: Journal Articles Status: Published Year Published: 2016 Citation: Miller B, G Anfora, M Buffington, KM Daane, DT Dalton, KM Hoelmer, MV Stacconi, A Grassi, C Ioriatti, A Loni, JC Miller, M. Quantar, X. Wang, NG Wiman, and VM Walton. 2015. Seasonal occurrence of resident parasitoids associated with Drosophila suzukii in two small fruit production regions of Italy and the USA. Bulletin of Insectology 68(2): 255-63.
12. Type: Journal Articles Status: Published Year Published: 2016 Citation: Shearer PW, J West, VM Walton, P Brown, N Svetec, and J Chiu. 2016. Environmental cues enhance winter survival of Drosophila suzukii. BMC Ecology. 16:11. DOI: 10.1186/s12898-016-0070-3.
13. Type: Journal Articles Status: Published Year Published: 2016 Citation: Tochen S, VM Walton and JC Lee. 2016. Impact of floral feeding on adult Drosophila suzukii survival and nutrient status. Journal of Pest Science.DOI: 10.1007/s10340-016-0762-7.
14. Type: Journal Articles Status: Published Year Published: 2016 Citation: Tochen S, JM Woltz, DT Dalton, JC Lee, NG Wiman, and VM Walton. 2015. Humidity affects populations of Drosophila suzukii (Diptera: Drosophilidae) in blueberry. Journal of Applied Entomology. doi: 10.1111/jen.12247.
15. Type: Journal Articles Status: Published Year Published: 2016 Citation: Wallingford A and GM Loeb. 2016. Developmental acclimation of Drosophila suzukii and its effect on diapause and winter stress tolerance. Environmental Entomology, DOI: 10.1093/ee/nvw088.
16. Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Walton VM, HJ Burrack, DT Dalton, R Isaacs, NG Wiman, and C Ioriatti. 2016. Past Present and future Drosophila suzukii distribution, impact and management in United States berry fruits. XXIX International Horticultural Congress: IHC2014. Location. Brisbane (Australia). Past, present and future of Drosophila suzukii: Distribution, impact and management in United States berry fruits. Acta Horticulturae DOI: 10.17660.Hort (ISHS).
17. Type: Journal Articles Status: Published Year Published: 2016 Citation: Wang XG, G Ka?ar, A Biondi, and KM Daane. 2016. Foraging efficiency and outcomes of interactions of two pupal parasitoids attacking spotted wing drosophila. Biological Control 96: 64-71.
18. Type: Journal Articles Status: Published Year Published: 2016 Citation: Wang XG, TG Stewart, A Biondi, BM Chavez, C Ingels, JA Caprile, J Grant, Walton VM, and KM Daane. 2016. Population dynamics and ecology of Drosophila suzukii in Central California. Journal of Pest Science. DOI: 10.1007/s10340-016-0747-6.
19. Type: Journal Articles Status: Published Year Published: 2016 Citation: Wiman N. G., Gianfranco Anfora, Antonio Biondi, Joanna C. Chiu, Kent M. Daane, Daniel T. Dalton, Beverly Gerdeman, Angela Gottardello, Kelly A. Hamby, Rufus Isaacs, Alberto Grassi, Claudio Ioriatti, Jana C. Lee, Betsey Miller, M. Valerio Rossi Stacconi, Peter W. Shearer, Lynell Tanigoshi, Xingeng Wang and V. M. Walton 2016. Drosophila suzukii population response to the environment and management strategies. J Pest Sci DOI: 10.1007/s10340-016-0757-4.
20. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Burrack, H.J., L.M. Diepenbrock, K. Swoboda-Bhattarai, Y. Zheng, and A. Sial. 29 September 2016. A season-long strategy to manage spotted wing drosophila. International Congress of Entomology. Orlando, FL.
21. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Sial, A. A. Packaging science & technology to deliver sustainable IPM programs for an invasive pest, spotted wing drosophila. In symposium ?Integrated Pest Management? at 90th Annual Meeting of Entomological Society of America Southeastern Branch, 13-16 March 2016, Raleigh, NC.
22. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Daane KM. Spotted wing drosophilas natural enemies and the importance of landscape ecology for classical biological control. XXV International Congress of Entomology, Orlando, FL, 25-30 Sept. 2016.
23. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Walton, V.M., N.G. Wiman, D.T. Dalton, B. Miller, R.M. York, P.W. Shearer, S.L. Tochen, L.K. Tanigoshi, B.S. Gerdeman, J.C. Lee, C. Ioriatti, G. Anfora, A. Grassi, R. Isaacs, and H.J. Burrack. 29 September 2016. Biology, management, and population modeling of Drosophila suzukii in western North American production regions. International Congress of Entomology. Orlando, FL.
24. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Wang XG, Biondi A, Miller B, Miller JC, Shearer PW, Hoelmer KA, Walton VM, Daane KM. Host range test of candidate Asian parasitoids for biological control of spotted wing drosophila in North America. XXV International Congress of Entomology, Orlando, FL, 25-30 Sept. 2016.
25. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Kent Daane, Xingeng Wang, Vaughn Walton, Emilio Guerrieri, Massimo Giorgini, Kim Alan Hoelmer. Control Biol?gico de Drosophila suzukii - Una plaga en ciernes para la fruticultura chilena (Biological Control of Drosophila suzukii, a Potential Pest for Chile), 3rd Symp. Chilean Soc. Biological Control, 30 August 2016, Chillan, Chile.
26. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Xiaoli Fan* and Miguel Gomez. Optimal Monitoring and Controlling of Invasive Species: The Case of Spotted Wing Drosophila in the United States. Annual Conference of the Agricultural and Applied Economics Association, Boston, August 1-3, 2016.
27. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Biondi A, Wang XG, Miller JC, Miller B, Shearer PW, Zappala L, Siscaro G, Hoelmer KA, Walton VM, Daane KM. Potential for importation of Asian parasitoids for the classical biological control of spotted wing Drosophila. XXV National Congress of Entomology of Italy, Padova, Italy, 20-24 June, 2016.
28. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Daane KM, Wang XG, Biondi A, Miller B, Miller JC, Shearer PW, Hoelmer KA, Walton VM.. Biological Control of Spotted Wing Drosophila - Is There Potential? 71st Annual Meeting of the ESA North Central Branch, Cleveland, Ohio, June 5-8, 2016.
29. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Hickner, P. 2016. The Making of a Pest: Insights From Chemosensation in Drosophila suzuki. 71st Annual Meeting of the North Central Branch of the Entomological Society of America. June 5-8, 2016. Cleveland, Ohio
30. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Evans, R. K. and A. A. Sial. Effect of abiotic factors on chronobiology of Drosophila suzukii (Diptera: Drosophilidae). 80th Annual Meeting of Georgia Entomological Society, Pine Mountain, GA. 6-8 April 2016.
31. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Grant, J. A. and A. A. Sial. Investigating wild flora for viable hosts of Drosophila suzukii (Diptera: Drosophilidae) in the Southeastern US. 80th Annual Meeting of Georgia Entomological Society, Pine Mountain, GA. 6-8 April 2016.
32. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Sial, A. A. Spotted wing drosophila management in Georgia blueberries. 80th Annual Meeting of Georgia Entomological Society, Pine Mountain, GA. 6-8 April 2016.
33. Type: Conference Papers and Presentations Status: Other Year Published: 2016 Citation: Kirkpatrick*, D., J. Miller and L Gut. Jan 2016. Orchard Pest and Disease Management Conference, Portland, OR. Improving Trap Efficiency and Retention for Spotted Wing Drosophila.
34. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Gautam, B. K. and Sial, A. A. Effect of temperature on acute toxicity of insecticides to spotted wing drosophila. The 63rd Annual Meeting of the ESA, 15-18 November 2015, Minneapolis, MN.
35. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Matthew Grieshop J. Huang, D. Kirkpatrick, L. Gut and R. Isaacs. Nov 2015. Entomol. Soc. Amer. annual meeting, Minneapolis, MN. Preliminary development of an insecticide based attract-and-kill tactic for spotted wind drosophila.
36. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: D. Kirkpatrick*, P. S. McGhee, S. Hermann, L. Gut and J. R. Miller. Alightment of spotted wing drosophila, Drosophila suzukii (Diptera: Drosophilidae), on disks with varying visual and olfactory stimuli. Nov 2015. Entomol. Soc. Amer. annual meeting, Minneapolis, MN.
37. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Little, B. A. and Sial, A. A. Effect of spray solution pH on efficacy and residual activity of insecticides against Drosophila suzukii (Diptera: Drosophilidae). The 63rd Annual Meeting of the ESA, 15-18 November 2015, Minneapolis, MN.
38. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Sial, A. A. 2015 Georgia state update on biology and management of of spotted wing drosophila. The 63rd Annual Meeting of the ESA, 15-18 November 2015, Minneapolis, MN.
39. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Sial, A. A. Issues surrounding management of spotted wing drosophila in southeastern United States. The 63rd Annual Meeting of the ESA, 15-18 November 2015, Minneapolis, MN.
40. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Walton V. M., Nik G. Wiman, Daniel T. Dalton, Kelly Hamby, Rufus Isaacs, Gianfranco Anfora, Joanna C. Chiu, Kent M. Daane, Alberto Grassi, Betsey Miller, Xingeng Wang and Claudio Ioriatti. Refined models for Spotted Wing Drosophila population estimation and management. WERA Symposium, Minneapolis, USA. November 15-18, 2015.
41. Type: Conference Papers and Presentations Status: Other Year Published: 2015 Citation: Walton V. M., N. G. Wiman, Daniel T. Dalton, Rufus Isaacs, Gianfranco Anfora, Joanna C. Chiu, Kent M. Daane, Alberto Grassi, Betsey Miller, Xingeng Wang and Claudio Ioriatti. Spotted Wing Drosophila population estimation and management. International IPM Symposium, Salt Lake City, USA. March 23-26, 2015.
42. Type: Websites Status: Published Year Published: 2016 Citation: Project website: SWDManagement.org
43. Type: Websites Status: Published Year Published: 2016 Citation: SWD News posts from NC State University Extension Portals: https://swd.ces.ncsu.edu/search-results/?q=swd

SUPPLEMENTARY DATA:  Institution Type: SAES  Coop States: NC  Region: 2  Process Date: 2015/08/21  Progress Update: 2020/01/07  Program Code: SCRI